MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
An international unit (IU) of enzyme activity is the quantity of
enzyme that:
a. Converts 1 umol of substrate to product per liter
b. Forms 1 mg of product per deciliter
c. Converts 1 umol of substrate to product per minute
d. Forms 1 umol of product per deciliter -
correct answer ✅c. Converts 1 umol of substrate to product per
minute
Which of the following measurement modes does not allow for
continuous monitoring of enzyme activity?
a. Initial absorbance is measured followed by a second reading after
5 minutes
b. Absorbance is measured at 10-second intervals for 100 seconds
c. Absorbance is monitored continuously for 1 minute using a chart
recorder
d. Reflectance is measured from a xenon source lamp pulsing at 60
Hz -
correct answer ✅a. Initial absorbance is measured followed by a
second reading after 5 minutes
,MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
Which of the following statements regarding enzymatic reactions is
true?
a. The enzyme shifts the equilibrium of the reaction to the right
b. The enzyme alters the equilibrium constant of the reaction
c. The enzyme increases the rate of the reaction
d. The enzyme alters the energy difference between reactants and
products -
correct answer ✅c. The enzyme increases the rate of the reaction
Which statement about enzymes is true?
a. An enzyme alters the Gibbs free energy of the reaction
b. Enzymes cause a reaction with a positive free energy to occur
spontaneously
c. An enzyme's natural substrate has the highest Km
d. A competitive inhibitor will alter the apparent Km of the reaction
-
correct answer ✅d. A competitive inhibitor will alter the apparent
Km of the reaction
Which substrate concentration is needed to achieve zero-order
condition?
,MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
a. Greater than 99 x Km
b. [S] = Km
c. Less than 10 x Km
d. [S] = 0 -
correct answer ✅a. Greater than 99 x Km
Which of the following statements is true?
a. Apoenzyme + prosthetic group = holoenzyme
b. A coenzyme is an inorganic molecule required for activity
c. Cofactors are as tightly bound to the enzyme as prosthetic groups
d. All enzymes have optimal activity at pH 7.00 -
correct answer ✅a. Apoenzyme + prosthetic group = holoenzyme
Which of the following statements about enzymatic reactions is
true?
a. NADH has absorbance maxima at 340 and 366 nm
b. Enzyme concentration must be in excess to achieve zero-order
kinetics
c. Rate is proportional to substrate concentration in a zero-order
reaction
, MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
d. Accumulation of the product increase the reaction rate -
correct answer ✅a. NADH has absorbance maxima at 340 and 366
nm
The increase in the level of serum enzymes used to detect
cholestatic liver disease is caused mainly by:
a. Enzyme release from dead cells
b. Leakage from cells with altered membrane permeability
c. Decreased perfusion of the tissue
d. Increased production and secretion by cells -
correct answer ✅d. Increased production and secretion by cells
Which of the following enzymes is considered most tissue specific?
a. CK
b. Amylase
c. ALP
d. ADH -
correct answer ✅d. ADH
Which of the following enzymes is activated by calcium ions?
Review Questions And Answers
An international unit (IU) of enzyme activity is the quantity of
enzyme that:
a. Converts 1 umol of substrate to product per liter
b. Forms 1 mg of product per deciliter
c. Converts 1 umol of substrate to product per minute
d. Forms 1 umol of product per deciliter -
correct answer ✅c. Converts 1 umol of substrate to product per
minute
Which of the following measurement modes does not allow for
continuous monitoring of enzyme activity?
a. Initial absorbance is measured followed by a second reading after
5 minutes
b. Absorbance is measured at 10-second intervals for 100 seconds
c. Absorbance is monitored continuously for 1 minute using a chart
recorder
d. Reflectance is measured from a xenon source lamp pulsing at 60
Hz -
correct answer ✅a. Initial absorbance is measured followed by a
second reading after 5 minutes
,MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
Which of the following statements regarding enzymatic reactions is
true?
a. The enzyme shifts the equilibrium of the reaction to the right
b. The enzyme alters the equilibrium constant of the reaction
c. The enzyme increases the rate of the reaction
d. The enzyme alters the energy difference between reactants and
products -
correct answer ✅c. The enzyme increases the rate of the reaction
Which statement about enzymes is true?
a. An enzyme alters the Gibbs free energy of the reaction
b. Enzymes cause a reaction with a positive free energy to occur
spontaneously
c. An enzyme's natural substrate has the highest Km
d. A competitive inhibitor will alter the apparent Km of the reaction
-
correct answer ✅d. A competitive inhibitor will alter the apparent
Km of the reaction
Which substrate concentration is needed to achieve zero-order
condition?
,MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
a. Greater than 99 x Km
b. [S] = Km
c. Less than 10 x Km
d. [S] = 0 -
correct answer ✅a. Greater than 99 x Km
Which of the following statements is true?
a. Apoenzyme + prosthetic group = holoenzyme
b. A coenzyme is an inorganic molecule required for activity
c. Cofactors are as tightly bound to the enzyme as prosthetic groups
d. All enzymes have optimal activity at pH 7.00 -
correct answer ✅a. Apoenzyme + prosthetic group = holoenzyme
Which of the following statements about enzymatic reactions is
true?
a. NADH has absorbance maxima at 340 and 366 nm
b. Enzyme concentration must be in excess to achieve zero-order
kinetics
c. Rate is proportional to substrate concentration in a zero-order
reaction
, MMSC436 Exam 1 Enzyme & Cardiac
Review Questions And Answers
d. Accumulation of the product increase the reaction rate -
correct answer ✅a. NADH has absorbance maxima at 340 and 366
nm
The increase in the level of serum enzymes used to detect
cholestatic liver disease is caused mainly by:
a. Enzyme release from dead cells
b. Leakage from cells with altered membrane permeability
c. Decreased perfusion of the tissue
d. Increased production and secretion by cells -
correct answer ✅d. Increased production and secretion by cells
Which of the following enzymes is considered most tissue specific?
a. CK
b. Amylase
c. ALP
d. ADH -
correct answer ✅d. ADH
Which of the following enzymes is activated by calcium ions?
