bio 2117 lab final Questions with 100% Verified Answers
Latest Update
Question: Catalase Test
Answer:
1) use loop to aseptically smear bacteria on clean glass slide
2) add 1-2 drops of hydrogen peroxide to each positive- will bubble negative- no change
differentiates gram positives.
Question: oxidase test
Answer:
1) get oxidase slide
2) use loop to get bacteria from culture and rub on slide
3) sterilize in between
4) observe after 20 secs positive- turns blue (obligate aerobe) negative- no change
differentiate between obligate aerobes and obligate anaerobes
Question:
Answer:
Question: amylase test
Answer:
amylase breaks down starch agar plate
1) use inoculating loop to zig zag bacteria on each half of plate
2) reagent used- LUGOL'S iodine solution positive- clear under bacteria negative-
brown/black under bacteria
Question: urease test
Answer:
1) obtain 2 urease tubes
1) stab and streak bacteria then stab and streak into urease tubes positive- pink bacteria
has the ability to create ammonia causes alkaline PH negative- yellow
Latest Update
Question: Catalase Test
Answer:
1) use loop to aseptically smear bacteria on clean glass slide
2) add 1-2 drops of hydrogen peroxide to each positive- will bubble negative- no change
differentiates gram positives.
Question: oxidase test
Answer:
1) get oxidase slide
2) use loop to get bacteria from culture and rub on slide
3) sterilize in between
4) observe after 20 secs positive- turns blue (obligate aerobe) negative- no change
differentiate between obligate aerobes and obligate anaerobes
Question:
Answer:
Question: amylase test
Answer:
amylase breaks down starch agar plate
1) use inoculating loop to zig zag bacteria on each half of plate
2) reagent used- LUGOL'S iodine solution positive- clear under bacteria negative-
brown/black under bacteria
Question: urease test
Answer:
1) obtain 2 urease tubes
1) stab and streak bacteria then stab and streak into urease tubes positive- pink bacteria
has the ability to create ammonia causes alkaline PH negative- yellow
