Medical Cell Biology (CH 01) Study Guide
visible light passes through specimens and lenses form light microscopy
an image
light microscopy uses [....] to magnify lenses
light microscopy can magnify up to 1000x
Advantages of light microscopy: Simple and inexpensive
Advantages of light microscopy: Allows [.....] imaging live-cell
Advantages of light microscopy: minimal sample preparation
limitations of light microscopy: resolution limited by diffraction (~200 nm)
Applications of light microscopy: cell [....] and cell [....] morphology and counting
Applications of light microscopy: tissue [...] histology
fluorophores absorb [....] light
Fluorophores absorb light at one wavelength and emit longer wavelength
light at a
Advantages of fluorescence microscopy: high specificity
Advantages of fluorescence microscopy: Visualize proteins or organelles
specific
Advantages of fluorescence microscopy: Live- cell imaging (possible)
Limitations of fluorescence microscopy: [....] and [....] photobleaching, phototoxicity
Limitations of fluorescence microscopy: resolution diffraction limited
, Medical Cell Biology (CH 01) Study Guide
Applications of fluorescence microscopy: protein [....] localization
Applications of fluorescence microscopy: [.....] [....] gene expression
studies
Advantages of fluorescence microscopy: tracking cellular processes
Labeling something specific with Fluorophores Fluorescence Microscopy
Uses laser scanning and pinhole apertures to eliminate confocal microscopy
out-of-focus light
Confocal Microscopy: uses [.....] and [....] to eliminate laser scanning and pinhole apertures
out-of-focus light
Confocal Microscopy: Uses laser scanning and pinhole out of focus light
apertures to eliminate
Advantages of Confocal Microscopy: higher contrast
Advantages of Confocal Microscopy: optical sectioning
Advantages of Confocal Microscopy: 3D reconstruction
Limitations of Confocal Microscopy: expensive, slower image acquisition, potential photo damage
Applications of Confocal Microscopy: thick tissues
Advantages of Confocal Microscopy: [...] localization intracellular
Advantages of Confocal Microscopy: [...] imaging 3D
Energy transfer occurs between two fluorophores only FRET
when they are within 1-10 nm
visible light passes through specimens and lenses form light microscopy
an image
light microscopy uses [....] to magnify lenses
light microscopy can magnify up to 1000x
Advantages of light microscopy: Simple and inexpensive
Advantages of light microscopy: Allows [.....] imaging live-cell
Advantages of light microscopy: minimal sample preparation
limitations of light microscopy: resolution limited by diffraction (~200 nm)
Applications of light microscopy: cell [....] and cell [....] morphology and counting
Applications of light microscopy: tissue [...] histology
fluorophores absorb [....] light
Fluorophores absorb light at one wavelength and emit longer wavelength
light at a
Advantages of fluorescence microscopy: high specificity
Advantages of fluorescence microscopy: Visualize proteins or organelles
specific
Advantages of fluorescence microscopy: Live- cell imaging (possible)
Limitations of fluorescence microscopy: [....] and [....] photobleaching, phototoxicity
Limitations of fluorescence microscopy: resolution diffraction limited
, Medical Cell Biology (CH 01) Study Guide
Applications of fluorescence microscopy: protein [....] localization
Applications of fluorescence microscopy: [.....] [....] gene expression
studies
Advantages of fluorescence microscopy: tracking cellular processes
Labeling something specific with Fluorophores Fluorescence Microscopy
Uses laser scanning and pinhole apertures to eliminate confocal microscopy
out-of-focus light
Confocal Microscopy: uses [.....] and [....] to eliminate laser scanning and pinhole apertures
out-of-focus light
Confocal Microscopy: Uses laser scanning and pinhole out of focus light
apertures to eliminate
Advantages of Confocal Microscopy: higher contrast
Advantages of Confocal Microscopy: optical sectioning
Advantages of Confocal Microscopy: 3D reconstruction
Limitations of Confocal Microscopy: expensive, slower image acquisition, potential photo damage
Applications of Confocal Microscopy: thick tissues
Advantages of Confocal Microscopy: [...] localization intracellular
Advantages of Confocal Microscopy: [...] imaging 3D
Energy transfer occurs between two fluorophores only FRET
when they are within 1-10 nm
