NHA PHLEBOTOMY CERTIFICATION Exam
2026/2027 QUESTIONS WITH ANSWERS
GRADED A+
• Normal Hemoglobin A1C Assay Result -✓✓ 4.0 and below
• Pre-diabetic Hemoglobin A1C Assay Result -✓✓ 7.0 and above
• Median Cubital Vein -✓✓ Most preferred for phlebotomy
• Select Lancet Size -✓✓ Phlemotomist should ____ prior to performing capillary
puncture
• Chain of Infection -✓✓ 1) Agent 2) Reservoir 3) Portal of Exit 4) Mode of Transmission
5) Portal of Entry 6) susceptible host
• Infectious Agents -✓✓ Viruses, Bacteria, Fungi, parasites
• Portal of Exit -✓✓ Agent leaves its reservoir
• Standard precautions (or transmission-based precautions) -✓✓ Control measures
aimed at preventing the spread of the disease as infectious agents exit the reservoir
• Blood Culture -✓✓ Checks for bacteria and septicemia
• 8-10 ML -✓✓ When obtaining a blood culture from an adult, you should collect _____
• Portal of entry -✓✓ Allows infectious agent access to susceptible host (broken skin,
mucous membranes, and body systems exposed to external environment such as
gastrointestinal, respiratory, and reproductive)
• Limit Transmission of infectious agent -✓✓ Transmission-based precautions (gloves,
mask), and aseptic technique (hand washing)
• Susceptible Host -✓✓ 'At risk', not resistant or immune
• Infection control at susceptible host level -✓✓ Identification of the patients at risk, treat
their underlying condition for susceptibility, or isolate them from the reservoir
, • Fasting Specimen -✓✓ Usually glucose, cholesterol, triglycerides, vitamins (anything
that has to do with food)
• Lavender Tube -✓✓ Pregnancy, hemoglobin A1C Assay
• Successful Smear -✓✓ Will have 'feathered edge'
• Stool Specimens -✓✓ Occult Blood (or 'hidden blood'), analyze for dietary products
and digestive secretions, decreased amount of bile in stool
• Guaiac Test -✓✓ Occult or 'hidden' blood in stool
• Steatorrhea -✓✓ Excessive amount of fat in stool- can indicate faulty absorption of fat
from small intestine
• Decreased amount of bile in stool -✓✓ Indicates obstruction in bile flow from liver and
gallbladder to intestine as well as gallbladder removal
• Labeling blood samples -✓✓ Must have patient name, ID(ULI Number), DOB, date and
time of collection, site location, collectors initials
• Stool and Urine Specimen labeling -✓✓ Label on container, not lid
• Finger Sticks (adults) -✓✓ Non-dominant hand, middle finger is preferred
• Finger sticks (children) -✓✓ Lateral portion of heel (if child is not walking for 1 year)
• Age and Development -✓✓ Factors that determine whether a child will have capillary
collection by heel or finger stick
• Detecting presence of ova and parasites in stool sample -✓✓ Send specimen as soon
as possible, if it will be seen in >24 hours, **ice sample (chill sample, put sample in
refrigerator)
• Testing feces for
Occult blood -✓✓ Use chemical reagent substance to detect presence of enzyme
peroxidase in hemoglobin molecule
• Guaiac Test Results -✓✓ Blue color- postive result
No color, or any color other than blue- negative result- absence of blood in stool)
• False Readings for Guaiac Test -✓✓ Certain foods, medications, vitamin C
False positive- recently ingested red meat, raw veg or fruit, or certain meds that cause
gastric bleeding (NSAID, aspirin, anticoagulants)
2026/2027 QUESTIONS WITH ANSWERS
GRADED A+
• Normal Hemoglobin A1C Assay Result -✓✓ 4.0 and below
• Pre-diabetic Hemoglobin A1C Assay Result -✓✓ 7.0 and above
• Median Cubital Vein -✓✓ Most preferred for phlebotomy
• Select Lancet Size -✓✓ Phlemotomist should ____ prior to performing capillary
puncture
• Chain of Infection -✓✓ 1) Agent 2) Reservoir 3) Portal of Exit 4) Mode of Transmission
5) Portal of Entry 6) susceptible host
• Infectious Agents -✓✓ Viruses, Bacteria, Fungi, parasites
• Portal of Exit -✓✓ Agent leaves its reservoir
• Standard precautions (or transmission-based precautions) -✓✓ Control measures
aimed at preventing the spread of the disease as infectious agents exit the reservoir
• Blood Culture -✓✓ Checks for bacteria and septicemia
• 8-10 ML -✓✓ When obtaining a blood culture from an adult, you should collect _____
• Portal of entry -✓✓ Allows infectious agent access to susceptible host (broken skin,
mucous membranes, and body systems exposed to external environment such as
gastrointestinal, respiratory, and reproductive)
• Limit Transmission of infectious agent -✓✓ Transmission-based precautions (gloves,
mask), and aseptic technique (hand washing)
• Susceptible Host -✓✓ 'At risk', not resistant or immune
• Infection control at susceptible host level -✓✓ Identification of the patients at risk, treat
their underlying condition for susceptibility, or isolate them from the reservoir
, • Fasting Specimen -✓✓ Usually glucose, cholesterol, triglycerides, vitamins (anything
that has to do with food)
• Lavender Tube -✓✓ Pregnancy, hemoglobin A1C Assay
• Successful Smear -✓✓ Will have 'feathered edge'
• Stool Specimens -✓✓ Occult Blood (or 'hidden blood'), analyze for dietary products
and digestive secretions, decreased amount of bile in stool
• Guaiac Test -✓✓ Occult or 'hidden' blood in stool
• Steatorrhea -✓✓ Excessive amount of fat in stool- can indicate faulty absorption of fat
from small intestine
• Decreased amount of bile in stool -✓✓ Indicates obstruction in bile flow from liver and
gallbladder to intestine as well as gallbladder removal
• Labeling blood samples -✓✓ Must have patient name, ID(ULI Number), DOB, date and
time of collection, site location, collectors initials
• Stool and Urine Specimen labeling -✓✓ Label on container, not lid
• Finger Sticks (adults) -✓✓ Non-dominant hand, middle finger is preferred
• Finger sticks (children) -✓✓ Lateral portion of heel (if child is not walking for 1 year)
• Age and Development -✓✓ Factors that determine whether a child will have capillary
collection by heel or finger stick
• Detecting presence of ova and parasites in stool sample -✓✓ Send specimen as soon
as possible, if it will be seen in >24 hours, **ice sample (chill sample, put sample in
refrigerator)
• Testing feces for
Occult blood -✓✓ Use chemical reagent substance to detect presence of enzyme
peroxidase in hemoglobin molecule
• Guaiac Test Results -✓✓ Blue color- postive result
No color, or any color other than blue- negative result- absence of blood in stool)
• False Readings for Guaiac Test -✓✓ Certain foods, medications, vitamin C
False positive- recently ingested red meat, raw veg or fruit, or certain meds that cause
gastric bleeding (NSAID, aspirin, anticoagulants)
