Lab 7
Gel electrophoresis is a powerful tool to help visualize dna and even smaller
things like proteins
What you do is chop them up and put them through this gel to measure how
big they are
So you separate these fragments based on size.
The purpose is for dna testing because we get your dna from our parents so
you can see who family members are and determine relatiosnhips
One of the most useful tool in a bio lab
How does it work
You take a piece of DNA and digest it with restriction enzymes( they chew it
up at known regions) then we subject it to pcr which multiples it so that you
can look at many copies
, )
Step 1 is to denaturation which kinda unwinds the double helix an dmakes it
straight so that we can get to it, then break the hydrogen bond in the
Gel electrophoresis is a powerful tool to help visualize dna and even smaller
things like proteins
What you do is chop them up and put them through this gel to measure how
big they are
So you separate these fragments based on size.
The purpose is for dna testing because we get your dna from our parents so
you can see who family members are and determine relatiosnhips
One of the most useful tool in a bio lab
How does it work
You take a piece of DNA and digest it with restriction enzymes( they chew it
up at known regions) then we subject it to pcr which multiples it so that you
can look at many copies
, )
Step 1 is to denaturation which kinda unwinds the double helix an dmakes it
straight so that we can get to it, then break the hydrogen bond in the