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BIO 230 QUESTIONS WITH ACTUAL ANSWERS

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BIO 230 QUESTIONS WITH ACTUAL ANSWERS

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Binds to degredation sequences in substrates . a ubiqutin molecules is then added to
lysine residue in the target. The way in which E3 is activated regulates protein
degredation. It is sometimes phosphorylated or allosterically binded by ligands or
proteins.


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E3




Unwinds DNA, phosphorylates Ser 5 of the RNAp's ctd and releases rna p from the
promoter.


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TFIIH

,Assist in mRNA quality control in the cytosol. eIF4E bind the 5' cap displacing the CBC
and the poly-A binding protein is bound by eIF4G. eIFs recruit the small ribosomal
complex which initiates translation at the first AUG downstrea of the 5' cap.


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Eukaryotic initiation factors+eIF4e, eIF4G




Plays a role in RNAi, interacting with argonaute and RISC proteins and follow the
miRNA route to destroy double stranded RNA.
siRNA regultes transcription interacting with argonaute and rna-induced transcription
silencing complex RITS.


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siRNA




Tags proteins to marge their folding development, protects surface proteins from
proteases and helps with some 3-d structures.


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Glycosylation




recognizes BRE elements in promoters, positions RNAP at he start site.


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, TFIIB




recruits coat proteins for COPI and clathrin vesicles.


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ARF GTPase




Glycosylation varieties found in soluble and transmembrane proteins. The n-linked
types have a precursor of of glucose, manose and n-acetylglusamine. Proper folding
is assisted when glucose is removed by glucosidase and mannose by mannosidase.


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O-linked and n-linked




Hydrophobics AAs at the n-terminus emerge from translation first. The ribosome
directs it co-translationally to the ER. It gies the SRP and ribosome high affinity to bind
each other


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ER signal sequence




Chloroplasts are penetrated with a different N-terminal signal sequence. The
chloroplast signal sequence is cleaved exposing a thylakoid signal sequence for

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