QUESTIONS AND CORRECT ANSWERS | ASCP CG CERTIFICATION
TEST | ASCP CYTOGENETICS TEST | NEW 2026/2027 UPDATE |
GRADED A+.
What is the best agent to use against mycoplasma? - ANSWER-BM
cyclin - a combination of two antibiotics (tiamulin and minocycline)
that effecticely targets mycoplasma growth
When harvesting peripheral blood cultures, asceptic technique no
longer matters following incubation with: - ANSWER-Colcemid -
mitotic spindle poison that halts cells at metaphase
When scoring FISH for DiGeorge syndrome, a tech notices the TUPLE1
signal is present, but one of the control ARSA probe signals is missing
in every cell. This result suggests __ ? - ANSWER-The patient has a
deletion of the ARSA region
What is the nomenclature on a patient with down syndrome referred
for a hematologic disorder where only trisomy 21 was found? -
ANSWER-47,XY,+21c[20]
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For successful harvest, each new batch of hypotonic solution should
be evaluated by: - ANSWERcomparing the new lot to a lot already
in use
,Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic
enzymes - break down tissues into single-cell suspensions
24 hours after culture initiation, a single dish from an amniotic fluid
turns cloudy. Which cultures should be disposed? - ANSWER-The
single cloudy dish only
CLIA 1988- (Clinical Laboratory Improvement Amendments)
stipulates that equipment must be recalibrated __ ? - ANSWER-
Every 6 months (Standard 493.1217)
What type of stained chromosome would produce a better computer
image? - ANSWER-Lightly stained chromosomes (best contrast and
can be digitally edited easier)
What is used to reduce mycoplasma contamination? - ANSWER-
BM cycline
CLIA '88 stipulates that equipment must be recalibrated ____ ? -
ANSWER-After a major repair to ensure still operational
, How should caustic and corrosive chemicals be stored? - ANSWER-
Both should be stored in a nonflammable cabinet
CBG = c-bands achieved with barium with Giemsa stain
CBG negative referrs to not having a centromere, which is crucial to
being included in the modal number, so this extra fragment does not
have a centromere and is not counted towards the total
What is included when recording the modal number? -
ANSWERMar - a marker chromosome is included because it has a
centromere
What chemical is used for cryopreservation? - ANSWER-Dimethyl
sulfoxide (DMSO)
What factors would contribute to ALL cultures failing in a CO2
incubator? - ANSWER-Temperature
CO2 level
Humidity level
What is the modal number for a diploid karyotype and an additional
CBG negative marker chromosome? - ANSWER-46