ALAT CERTIFICATION FINAL EXAM LATEST 2026/ 2027 QUESTIONS AND ANSWERS (REAL EXAM
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
1. Which of the following species is covered by the Animal Welfare Act?
• Options:
o Birds bred specifically for research
o Zebrafish
o Mice
o Hamsters
• Correct Answer: Hamsters
2. What types of euthanasia methods are listed in the AVMA Guidelines for the Euthanasia
of Animals?
• Options:
o Suitable, conditionally suitable, and unsuitable methods
o Adequate, acceptable, and inadequate methods
o Acceptable, conditionally acceptable, and unacceptable methods
o Approved, tentative, and unapproved methods
• Correct Answer: Acceptable, conditionally acceptable, and unacceptable methods
3. The PHS Policy covers all live vertebrates used or intended for use in research, training,
experimental purposes and biological testing.
• Options:
o False
o True
• Correct Answer: True
4. Which document on animal research is reviewed and approved by the Institutional
Animal Care and Use Committee?
• Options:
o Research proposal
o Grant
o Animal use protocol
o Research data report
• Correct Answer: Animal use protocol
5. What federal agency is responsible for enforcing and administering the Animal Welfare
Regulations?
• Options:
o Animal and Plant Health Inspection Service
o Food and Drug Administration
o Office of Laboratory Animal Welfare
o AAALAC International
• Correct Answer: Animal and Plant Health Inspection Service
,ALAT CERTIFICATION FINAL EXAM LATEST 2026/ 2027 QUESTIONS AND ANSWERS (REAL EXAM
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
6. What is NOT a requirement of the AWA/AWR a researcher must comply with, if they
house covered species?
• Options:
o Appoint an unaffiliated member to the IACUC.
o Submit an annual report including the number of covered species used.
o Provide dogs with exercise.
o Meet or exceed sanitization standards for both cages and facility.
• Correct Answer: Appoint an unaffiliated member to the IACUC.
7. According to the Animal Welfare Regulations, to which official does the IACUC report?
• Options:
o The Institutional Official (IO) or Chief Executive Officer (CEO)
o The Chair of the Office of Laboratory Animal Welfare or the Area Director
o The Director of the USDA Animal & Plant Health Inspection Service or Chief
Inspecting Officer
o The Attending Veterinarian or Facility Director
• Correct Answer: The Institutional Official (IO) or Chief Executive Officer (CEO)
8. Which agency is responsible for enforcing the Public Health Service Policy on Humane
Care and Use of Laboratory Animals (PHS Policy)?
• Options:
o Food and Drug Administration
o Animal and Plant Health Inspection Service
o Office of Laboratory Animal Welfare
o National Research Council
• Correct Answer: Office of Laboratory Animal Welfare
9. What is NOT stated as a requirement for compliance with the US Principles for the
Utilization and Care of Vertebrate Animals Used in Testing, Research and Training?
• Options:
o Transportation, use and care of animals should be in accordance with the Animal
Welfare Act.
o Principal investigators should determine where exceptions to these principles are
required and may ignore them if they decide it is a scientific necessity.
o Procedures that cause more than momentary or slight pain must be performed
with appropriate sedation, analgesia and anesthesia.
o Animals that suffer unrelieved pain and distress should be painlessly killed at the
end of the procedure.
• Correct Answer: Principal investigators should determine where exceptions to these
principles are required and may ignore them if they decide it is a scientific necessity.
, ALAT CERTIFICATION FINAL EXAM LATEST 2026/ 2027 QUESTIONS AND ANSWERS (REAL EXAM
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
10. What is the term for the field of study concerned with the philosophical issues raised by
some medical procedures and treatments?
• Options:
o The 3Rs
o Bioethics
o Animal welfare
o Biotechnology
• Correct Answer: Bioethics
11. What organization certifies laboratory animal technicians at the ALAT, LAT, and
LATG levels?
• Options:
o A) USDA
o B) AALAS (American Association for Laboratory Animal Science)
o C) OLAW
o D) NABR
• Correct Answer: B) AALAS (American Association for Laboratory Animal Science)
12. Which document is commonly referred to as the "Guide" in laboratory animal
research?
• Options:
o A) The Animal Welfare Act
o B) Guide for the Care and Use of Laboratory Animals
o C) The AVMA Guidelines on Euthanasia
o D) PHS Policy Manual
• Correct Answer: B) Guide for the Care and Use of Laboratory Animals
13. What is the minimum frequency with which the IACUC must inspect animal facilities
under the Animal Welfare Act and PHS Policy?
• Options:
o A) Monthly
o B) Every 6 months
o C) Annually
o D) Every 2 years
• Correct Answer: B) Every 6 months
14. What does the acronym "IACUC" stand for?
• Options:
o A) Institutional Animal Care and Use Committee
o B) International Animal Control and Usage Commission
o C) Institute for Animal Care and Universal Control
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
1. Which of the following species is covered by the Animal Welfare Act?
• Options:
o Birds bred specifically for research
o Zebrafish
o Mice
o Hamsters
• Correct Answer: Hamsters
2. What types of euthanasia methods are listed in the AVMA Guidelines for the Euthanasia
of Animals?
• Options:
o Suitable, conditionally suitable, and unsuitable methods
o Adequate, acceptable, and inadequate methods
o Acceptable, conditionally acceptable, and unacceptable methods
o Approved, tentative, and unapproved methods
• Correct Answer: Acceptable, conditionally acceptable, and unacceptable methods
3. The PHS Policy covers all live vertebrates used or intended for use in research, training,
experimental purposes and biological testing.
• Options:
o False
o True
• Correct Answer: True
4. Which document on animal research is reviewed and approved by the Institutional
Animal Care and Use Committee?
• Options:
o Research proposal
o Grant
o Animal use protocol
o Research data report
• Correct Answer: Animal use protocol
5. What federal agency is responsible for enforcing and administering the Animal Welfare
Regulations?
• Options:
o Animal and Plant Health Inspection Service
o Food and Drug Administration
o Office of Laboratory Animal Welfare
o AAALAC International
• Correct Answer: Animal and Plant Health Inspection Service
,ALAT CERTIFICATION FINAL EXAM LATEST 2026/ 2027 QUESTIONS AND ANSWERS (REAL EXAM
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
6. What is NOT a requirement of the AWA/AWR a researcher must comply with, if they
house covered species?
• Options:
o Appoint an unaffiliated member to the IACUC.
o Submit an annual report including the number of covered species used.
o Provide dogs with exercise.
o Meet or exceed sanitization standards for both cages and facility.
• Correct Answer: Appoint an unaffiliated member to the IACUC.
7. According to the Animal Welfare Regulations, to which official does the IACUC report?
• Options:
o The Institutional Official (IO) or Chief Executive Officer (CEO)
o The Chair of the Office of Laboratory Animal Welfare or the Area Director
o The Director of the USDA Animal & Plant Health Inspection Service or Chief
Inspecting Officer
o The Attending Veterinarian or Facility Director
• Correct Answer: The Institutional Official (IO) or Chief Executive Officer (CEO)
8. Which agency is responsible for enforcing the Public Health Service Policy on Humane
Care and Use of Laboratory Animals (PHS Policy)?
• Options:
o Food and Drug Administration
o Animal and Plant Health Inspection Service
o Office of Laboratory Animal Welfare
o National Research Council
• Correct Answer: Office of Laboratory Animal Welfare
9. What is NOT stated as a requirement for compliance with the US Principles for the
Utilization and Care of Vertebrate Animals Used in Testing, Research and Training?
• Options:
o Transportation, use and care of animals should be in accordance with the Animal
Welfare Act.
o Principal investigators should determine where exceptions to these principles are
required and may ignore them if they decide it is a scientific necessity.
o Procedures that cause more than momentary or slight pain must be performed
with appropriate sedation, analgesia and anesthesia.
o Animals that suffer unrelieved pain and distress should be painlessly killed at the
end of the procedure.
• Correct Answer: Principal investigators should determine where exceptions to these
principles are required and may ignore them if they decide it is a scientific necessity.
, ALAT CERTIFICATION FINAL EXAM LATEST 2026/ 2027 QUESTIONS AND ANSWERS (REAL EXAM
) 200 QUESTIONS AND ANSWERS (VERIFIED ANSWERS)
10. What is the term for the field of study concerned with the philosophical issues raised by
some medical procedures and treatments?
• Options:
o The 3Rs
o Bioethics
o Animal welfare
o Biotechnology
• Correct Answer: Bioethics
11. What organization certifies laboratory animal technicians at the ALAT, LAT, and
LATG levels?
• Options:
o A) USDA
o B) AALAS (American Association for Laboratory Animal Science)
o C) OLAW
o D) NABR
• Correct Answer: B) AALAS (American Association for Laboratory Animal Science)
12. Which document is commonly referred to as the "Guide" in laboratory animal
research?
• Options:
o A) The Animal Welfare Act
o B) Guide for the Care and Use of Laboratory Animals
o C) The AVMA Guidelines on Euthanasia
o D) PHS Policy Manual
• Correct Answer: B) Guide for the Care and Use of Laboratory Animals
13. What is the minimum frequency with which the IACUC must inspect animal facilities
under the Animal Welfare Act and PHS Policy?
• Options:
o A) Monthly
o B) Every 6 months
o C) Annually
o D) Every 2 years
• Correct Answer: B) Every 6 months
14. What does the acronym "IACUC" stand for?
• Options:
o A) Institutional Animal Care and Use Committee
o B) International Animal Control and Usage Commission
o C) Institute for Animal Care and Universal Control
