CLC Assessment Test/ Knowledge Test Questions with
Correct Verified Answers /Latest Version 2026.
Question 1: What are the two major hormones of lactation
and where are they secreted from?
A) Estrogen and Progesterone from the ovaries
B) Oxytocin and Prolactin from the pituitary gland
C) Human Placental Lactogen and Prolactin from the placenta
D) Oxytocin and Estrogen from the hypothalamus
Answer: B
Rationale: Oxytocin and prolactin are the two major hormones of
lactation, both secreted from the pituitary gland. Prolactin is
responsible for milk production, while oxytocin is responsible for
milk ejection (the let-down reflex). These hormones work together
to establish and maintain lactation.
1
,Question 2: Which cells are responsible for producing milk?
A) Myoepithelial cells
B) Alveolar cells
C) Ductal cells
D) Sebaceous cells
Answer: B
Rationale: Alveolar cells (also called lactocytes) are the milk-
producing cells within the breast. They form a layer of cells with
milk inside, surrounded by myoepithelial cells. The myoepithelial
cells contract when stimulated by oxytocin to eject milk from the
alveoli through the ducts.
Question 3: What happens when oxytocin hits myoepithelial
cells?
A) The cells stop producing milk
B) The cells start retracting, causing milk ejection
2
,C) The cells increase in size
D) The cells divide to create more milk
Answer: B
Rationale: When oxytocin binds to receptors on myoepithelial
cells, these cells contract and retract, squeezing milk from the
alveoli into the ducts. This is the "let-down reflex" or milk ejection
reflex. Oxytocin also helps ducts increase in size while the lumen
(opening) gets smaller, facilitating milk flow toward the nipple.
Question 4: How many nipple pores does each breast
typically have?
A) 1-2
B) 3-5
C) 6-10
D) 10-15
Answer: B
3
, Rationale: Each breast typically has 3-5 nipple pores (also called
nipple openings). Milk flows through these openings from the
collecting ducts during feeding. The number varies among women
but is generally in this range.
Question 5: What is the function of the substance secreted by
sebaceous glands on the areola?
A) To lubricate the nipple for easier latching
B) To provide antimicrobial protection and create a sticky
surface
C) To produce milk for the infant
D) To stimulate the let-down reflex
Answer: B
Rationale: The substance secreted by sebaceous glands
(Montgomery's glands) on the areola is both antimicrobial and
sticky. It provides protection against infection and helps create a
seal between the infant's mouth and the breast during feeding.
4
Correct Verified Answers /Latest Version 2026.
Question 1: What are the two major hormones of lactation
and where are they secreted from?
A) Estrogen and Progesterone from the ovaries
B) Oxytocin and Prolactin from the pituitary gland
C) Human Placental Lactogen and Prolactin from the placenta
D) Oxytocin and Estrogen from the hypothalamus
Answer: B
Rationale: Oxytocin and prolactin are the two major hormones of
lactation, both secreted from the pituitary gland. Prolactin is
responsible for milk production, while oxytocin is responsible for
milk ejection (the let-down reflex). These hormones work together
to establish and maintain lactation.
1
,Question 2: Which cells are responsible for producing milk?
A) Myoepithelial cells
B) Alveolar cells
C) Ductal cells
D) Sebaceous cells
Answer: B
Rationale: Alveolar cells (also called lactocytes) are the milk-
producing cells within the breast. They form a layer of cells with
milk inside, surrounded by myoepithelial cells. The myoepithelial
cells contract when stimulated by oxytocin to eject milk from the
alveoli through the ducts.
Question 3: What happens when oxytocin hits myoepithelial
cells?
A) The cells stop producing milk
B) The cells start retracting, causing milk ejection
2
,C) The cells increase in size
D) The cells divide to create more milk
Answer: B
Rationale: When oxytocin binds to receptors on myoepithelial
cells, these cells contract and retract, squeezing milk from the
alveoli into the ducts. This is the "let-down reflex" or milk ejection
reflex. Oxytocin also helps ducts increase in size while the lumen
(opening) gets smaller, facilitating milk flow toward the nipple.
Question 4: How many nipple pores does each breast
typically have?
A) 1-2
B) 3-5
C) 6-10
D) 10-15
Answer: B
3
, Rationale: Each breast typically has 3-5 nipple pores (also called
nipple openings). Milk flows through these openings from the
collecting ducts during feeding. The number varies among women
but is generally in this range.
Question 5: What is the function of the substance secreted by
sebaceous glands on the areola?
A) To lubricate the nipple for easier latching
B) To provide antimicrobial protection and create a sticky
surface
C) To produce milk for the infant
D) To stimulate the let-down reflex
Answer: B
Rationale: The substance secreted by sebaceous glands
(Montgomery's glands) on the areola is both antimicrobial and
sticky. It provides protection against infection and helps create a
seal between the infant's mouth and the breast during feeding.
4
