BIOD 171 MICROBIOLOGY MODULE 3
PORTAGE LEARNING REVISION GUIDE WITH
MICROSCOPY AND STAINING REVIEW
◉ Electron Microscopy.
Answer: uses electrons w/ specimens in a vacuum, Magnification:
(1,000x-1000,000x); Resolution:(1mm-0.1nm), Prokaryotic cells,
viruses, macromolecule and large atoms
◉ Probe Microscopy.
Answer: utilizes electronic probes that move over specimen service,
magnification: (>than1,000,000,000X); resolution:(0.01nm-10nm),
3D surface of macromolecules and atoms
◉ Bright field Microscope.
Answer: Uses: count microorganisms or cells, view stained specimen
view live unstained specimen, Features: staining usually required,
Types of Images: Bright background w/ clear or color specimen
◉ Phase Contrast Microscope.
Answer: Uses: view internal structures of live specimen, observe
motility(cilia and flagella), Features: No staining necessary so we
, can view live specimens, Types of Images: Image shows light and
dark areas of microbe
◉ Dark Microscope.
Answer: Uses: view living, unstained specimens, Features: Filter
inhibits light from going through organism and instead light is
reflected by organism, Types of images: Dark background w/ bright
specimen
◉ Fluorescence Microscopy.
Answer: Uses: localize specific structures or molecules, diagnostic
tool. features: uses UV light to excite fluorophores, visualize whole
cells specific structures, or proteins and watch movement or
interactions; Types of Images: dark background w/ fluorescence
structures
◉ Immunofluorescence.
Answer: These dyes are linked to antibodies which find a cellular
target and bind to it bringing the dye that can visualize and locate
the structure
◉ Confocal (Laser Scanning).
Answer: Uses: highly detailed structures, 3D renderings, Biofilms;
Features: Uses a laser to focus plane by plane through the specimen,
Types of Images: Single plain of structures with fluorescence dyes
PORTAGE LEARNING REVISION GUIDE WITH
MICROSCOPY AND STAINING REVIEW
◉ Electron Microscopy.
Answer: uses electrons w/ specimens in a vacuum, Magnification:
(1,000x-1000,000x); Resolution:(1mm-0.1nm), Prokaryotic cells,
viruses, macromolecule and large atoms
◉ Probe Microscopy.
Answer: utilizes electronic probes that move over specimen service,
magnification: (>than1,000,000,000X); resolution:(0.01nm-10nm),
3D surface of macromolecules and atoms
◉ Bright field Microscope.
Answer: Uses: count microorganisms or cells, view stained specimen
view live unstained specimen, Features: staining usually required,
Types of Images: Bright background w/ clear or color specimen
◉ Phase Contrast Microscope.
Answer: Uses: view internal structures of live specimen, observe
motility(cilia and flagella), Features: No staining necessary so we
, can view live specimens, Types of Images: Image shows light and
dark areas of microbe
◉ Dark Microscope.
Answer: Uses: view living, unstained specimens, Features: Filter
inhibits light from going through organism and instead light is
reflected by organism, Types of images: Dark background w/ bright
specimen
◉ Fluorescence Microscopy.
Answer: Uses: localize specific structures or molecules, diagnostic
tool. features: uses UV light to excite fluorophores, visualize whole
cells specific structures, or proteins and watch movement or
interactions; Types of Images: dark background w/ fluorescence
structures
◉ Immunofluorescence.
Answer: These dyes are linked to antibodies which find a cellular
target and bind to it bringing the dye that can visualize and locate
the structure
◉ Confocal (Laser Scanning).
Answer: Uses: highly detailed structures, 3D renderings, Biofilms;
Features: Uses a laser to focus plane by plane through the specimen,
Types of Images: Single plain of structures with fluorescence dyes
