BIOCHEM ACS CERTIFICATION
EVALUATION 2026 STUDY GUIDE
QUESTIONS AND ANSWERS
◉ The electron transport chain occurs in
Answer: The inner mitochondrial membrane
◉ Most ATP during aerobic respiration is produced during
Answer: Oxidative phosphorylation
◉ Oxygen functions as
Answer: The final electron acceptor in the electron transport chain
◉ Without oxygen
Answer: NADH accumulates because it cannot be efficiently oxidized
◉ Fermentation regenerates
Answer: NAD+
◉ The purpose of fermentation
Answer: Allow glycolysis to continue under low oxygen conditions
, ◉ Glycolysis directly produces
Answer: ATP and NADH
◉ The citric acid cycle mainly produces
Answer: NADH and FADH2
◉ Oxidative phosphorylation uses
Answer: NADH and FADH2 to generate ATP
◉ ATP synthase produces ATP using
Answer: A proton gradient
◉ Substrate-level phosphorylation directly produces
Answer: ATP
◉ Oxidative phosphorylation requires
Answer: Oxygen
◉ Fermentation produces much less ATP than
Answer: Aerobic respiration
EVALUATION 2026 STUDY GUIDE
QUESTIONS AND ANSWERS
◉ The electron transport chain occurs in
Answer: The inner mitochondrial membrane
◉ Most ATP during aerobic respiration is produced during
Answer: Oxidative phosphorylation
◉ Oxygen functions as
Answer: The final electron acceptor in the electron transport chain
◉ Without oxygen
Answer: NADH accumulates because it cannot be efficiently oxidized
◉ Fermentation regenerates
Answer: NAD+
◉ The purpose of fermentation
Answer: Allow glycolysis to continue under low oxygen conditions
, ◉ Glycolysis directly produces
Answer: ATP and NADH
◉ The citric acid cycle mainly produces
Answer: NADH and FADH2
◉ Oxidative phosphorylation uses
Answer: NADH and FADH2 to generate ATP
◉ ATP synthase produces ATP using
Answer: A proton gradient
◉ Substrate-level phosphorylation directly produces
Answer: ATP
◉ Oxidative phosphorylation requires
Answer: Oxygen
◉ Fermentation produces much less ATP than
Answer: Aerobic respiration