MICROBIOLOGY 2117 UPDATED FINAL EXAMS ALL
QUESTIONS AND ANSWERS SURE A+
✔✔three doman system - ✔✔BAE
Bacteria
Archaea
Eukarya
✔✔taxonomic scheme is further categorized into - ✔✔pcofgs
phylum (large group of related animals and plants)
class
order
family
genus
species
✔✔viruses - ✔✔acellular (no cell)
✔✔colony - ✔✔several individual organisms living together on close association
✔✔what do you use to obtain single colonies of bacteria - ✔✔streak plates
, ✔✔difference between a colony and the culture - ✔✔colony - isolated group of
microorganisms grown in a surface
culture - can be solid (agar) or liquid (broth) we used to grow microorganisms...it's the
medium
✔✔mixed culture - ✔✔more than one type of organisms growing in a sterile medium as
such as agar. Include multiple species
✔✔pure culture - ✔✔only one type of organisms growing in a sterile medium
✔✔difference between broth and solid media - ✔✔broth - liquid
solid - solid nutrients
✔✔why is agar used for solid media - ✔✔microbes cannot digest agar, therefore agar
media remain solid
✔✔defined media - ✔✔has exact amount of specific nutrients
maybe used to grow fastidious organisms
✔✔fastidious organisms - ✔✔organisms that require specific nutrients to grow.
they need large number of growth factors
✔✔complex media - ✔✔exact chemical composition is unknown
✔✔thioglycollate (anaerobic growth media) - ✔✔remove the free oxygen from the media
✔✔selective media - ✔✔contains substances that favor the growth of the organisms or
inhibit growth of unwanted organisms
✔✔differential media - ✔✔differences of appearance of colonies
e,g : blood agar
✔✔enhancement (enrichment media) - ✔✔use selective media.
can observe small number or chosen organism
✔✔when might someone do a streak plate in a clinical setting? - ✔✔to obtain single
colonies of bacteria
✔✔storing bacteria - refrigeration - ✔✔good for short period
✔✔lyophilization - ✔✔freeze drying
good for long period
QUESTIONS AND ANSWERS SURE A+
✔✔three doman system - ✔✔BAE
Bacteria
Archaea
Eukarya
✔✔taxonomic scheme is further categorized into - ✔✔pcofgs
phylum (large group of related animals and plants)
class
order
family
genus
species
✔✔viruses - ✔✔acellular (no cell)
✔✔colony - ✔✔several individual organisms living together on close association
✔✔what do you use to obtain single colonies of bacteria - ✔✔streak plates
, ✔✔difference between a colony and the culture - ✔✔colony - isolated group of
microorganisms grown in a surface
culture - can be solid (agar) or liquid (broth) we used to grow microorganisms...it's the
medium
✔✔mixed culture - ✔✔more than one type of organisms growing in a sterile medium as
such as agar. Include multiple species
✔✔pure culture - ✔✔only one type of organisms growing in a sterile medium
✔✔difference between broth and solid media - ✔✔broth - liquid
solid - solid nutrients
✔✔why is agar used for solid media - ✔✔microbes cannot digest agar, therefore agar
media remain solid
✔✔defined media - ✔✔has exact amount of specific nutrients
maybe used to grow fastidious organisms
✔✔fastidious organisms - ✔✔organisms that require specific nutrients to grow.
they need large number of growth factors
✔✔complex media - ✔✔exact chemical composition is unknown
✔✔thioglycollate (anaerobic growth media) - ✔✔remove the free oxygen from the media
✔✔selective media - ✔✔contains substances that favor the growth of the organisms or
inhibit growth of unwanted organisms
✔✔differential media - ✔✔differences of appearance of colonies
e,g : blood agar
✔✔enhancement (enrichment media) - ✔✔use selective media.
can observe small number or chosen organism
✔✔when might someone do a streak plate in a clinical setting? - ✔✔to obtain single
colonies of bacteria
✔✔storing bacteria - refrigeration - ✔✔good for short period
✔✔lyophilization - ✔✔freeze drying
good for long period
