ALAT Unit 2
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, List the three most common types of cage washers used tunnel washer; rack washer; cabinet washer
in today's animal facilities.
How does a RODAC plate test the effectiveness of a It can be inoculated with a sample taken from the environment and incubated.
sanitization program? Then, any growth of microorganisms can be observed
List two reasons that automatic watering systems are to provide a filter system for the water; eliminate ergonomic hazards of handling
often used to provide drinking water for laboratory water bottles
animals.
What features of a biological safety cabinet protect the A HEPA filter
animals inside?
How does a biological safety cabinet protect the people The air within the cabinet does not exit through the front opening; the cabinet air
using it from any pathogens or allergens the animals is filtered through a HEPA filter before it is exhausted into the room
inside of it may have?
What is one dangerous outcome of autoclaving a liquid Liquid will expand and it will explode
in a bottle that is tightly sealed and filled to the top?
To properly autoclave a material, the temperature and type of material; size of material; level of contamination of the material
pressure within the autoclave must be maintained for a
period of time indicated in the SOP. What three
characteristics of the material affect this required time?
List the three methods that are used to check the Chemical: autoclave tape; Biological: spore strips; Mechanical: checking gauges
effectiveness of an autoclave cycle, and give an example on the autoclave
of each.
Function: Class III BSC Protects the user from exposure to pathogenic microorganisms
Function: Incinerator Burns material such as animal tissues, trash
Function: Fume Hood Protects the user from chemical fumes
Function: Autoclave Sterilizes instruments, equipment, and glass items using superheated steam &
pressure
Function: Balance Uses a sliding beam and weighing platform to determine weights
What two features of a cage dumping station help protect The air circulating fan and HEPA filter
the technician during cage changing?
What chemicals are often added to animals' drinking Acid and chlorine
water to reduce bacterial contamination?
What is the primary reason that dirty cages and other Airborn dust and microorganisms can contaminate the animal room environment
soiled equipment should be take to the cage wash area
for cleaning instead of being hand cleaned in the animal
room?