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Exam Structure
Total Questions: 200
Answer Format: Answer in bold italics, explanation in italics
Time Recommendation: 3–4 hours (timed practice) or self-paced review
Content Distribution
Module 1 – Introduction to Biochemistry: Q1–Q25 (25 questions)
Module 2 – Biomolecules: Carbohydrates & Lipids: Q26–Q50 (25 questions)
Module 3 – Proteins & Nucleic Acids: Q51–Q75 (25 questions)
Module 4 – Enzyme Kinetics & Regulation: Q76–Q100 (25 questions)
Module 5 – Bioenergetics: Q101–Q125 (25 questions)
Module 6 – Glycolysis & Fermentation: Q126–Q150 (25 questions)
Module 7 – Citric Acid Cycle & Oxidative Phosphorylation: Q151–Q175 (25
questions)
Module 8 – Lipid & Amino Acid Metabolism + Integrated/Final: Q176–Q200 (25
questions)
Scoring Guide (Self-Assessment)
Correct Answer: 1 point
Incorrect/Blank: 0 points
Total Possible: 200 points
Module 1 – Introduction to Biochemistry (Q1–25)
Q1
,Which of the following best defines biochemistry?
A. The study of chemical reactions in nonliving systems
B. The study of the chemical processes within and relating to living organisms
C. The study of the structure of inorganic compounds
D. The study of physical properties of gases
E. The study of geological processes
B. The study of the chemical processes within and relating to living organisms
Biochemistry focuses on the molecules and reactions that underlie life processes.
Q2
Which functional group is characterized by a carbonyl group bonded to a hydroxyl group (–
COOH)?
A. Amino
B. Carboxyl
C. Phosphate
D. Hydroxyl
E. Methyl
B. Carboxyl
The carboxyl group (–COOH) is found in amino acids and fatty acids.
Q3
Which of the following is a key difference between prokaryotic and eukaryotic cells?
,A. Prokaryotes have membrane-bound organelles
B. Eukaryotes lack a nucleus
C. Eukaryotes have membrane-bound organelles and a nucleus
D. Prokaryotes contain mitochondria
E. Eukaryotes have circular DNA only
C. Eukaryotes have membrane-bound organelles and a nucleus
Eukaryotic cells contain a nucleus and organelles such as mitochondria and ER;
prokaryotes do not.
Q4
A solution with a pH of 3 has how many times more hydrogen ions than a solution with a pH
of 5?
A. 2 times
B. 10 times
C. 20 times
D. 100 times
E. 1,000 times
D. 100 times
pH is logarithmic; each unit change represents a 10-fold change in [H⁺].
Q5
Which of the following is the primary role of buffers in biological systems?
, A. Increase temperature
B. Maintain a relatively constant pH
C. Increase reaction rates
D. Store energy
E. Transport oxygen
B. Maintain a relatively constant pH
Buffers resist changes in pH upon addition of acid or base.
Q6
Which of the following is the most abundant molecule in living cells?
A. Protein
B. Lipid
C. Carbohydrate
D. Water
E. Nucleic acid
D. Water
Water makes up ~70% of cell mass and is essential for biochemical reactions.
Q7
Which of the following is a polymer of glucose used for energy storage in plants?
A. Glycogen
B. Cellulose
C. Starch