AACE CCT Primer EXAMS Test A+
Questions and Answers Verified Solutions
Latest Update 2026/2027
Answer:
specifies the exact DNA product to be amplified
●● How do primers work with or for DNA polymerase?
Answer:
primers serve as an extension point for DNA polymerase to build upon
●● when will annealing occur between primers and target DNA?
Answer:
when the complementary sequence matches up A & G, T & C
●● How does DNA polymerase extend the primers?
Answer:
by adding nucleotides to the developing DNA strand
●● Why do you want the GC content level to be < 50%?
Answer:
Most housekeeping genes and tumor-suppressor genes are GC wish, thus the DNA is less amenable
to amplification, also a lot of stop codons are GC rich
●● what is a primer dimer?
Answer:
molecules that have hybridized to each other due to strings of complementary bases in the primers
●● what are the causes of a primer dimer?
Answer:
Questions and Answers Verified Solutions
Latest Update 2026/2027
Answer:
specifies the exact DNA product to be amplified
●● How do primers work with or for DNA polymerase?
Answer:
primers serve as an extension point for DNA polymerase to build upon
●● when will annealing occur between primers and target DNA?
Answer:
when the complementary sequence matches up A & G, T & C
●● How does DNA polymerase extend the primers?
Answer:
by adding nucleotides to the developing DNA strand
●● Why do you want the GC content level to be < 50%?
Answer:
Most housekeeping genes and tumor-suppressor genes are GC wish, thus the DNA is less amenable
to amplification, also a lot of stop codons are GC rich
●● what is a primer dimer?
Answer:
molecules that have hybridized to each other due to strings of complementary bases in the primers
●● what are the causes of a primer dimer?
Answer:
