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Exam (elaborations)

MicroBiology 3407 Lab Final Exam | Questions & Complete Solutions

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This complete solutions guide for Microbiology 3407 Lab Final covers Gram staining, aseptic technique, media types, serial dilutions, Kirby-Bauer method, and biochemical tests — essential for microbiology students preparing for their lab final exam.

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Institution
Microbiology
Course
Microbiology

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MICROBIOLOGY 3407 LAB FINAL
EXAM QUESTIONS WITH COMPLETE
SOLUTIONS.
Q: Can media be both selective and differential?
- correct answer - Yes.


Q: How do you calculate original concentration? -
correct answer - Colonies × dilution factor.


Q: How do you calculate total magnification? -
correct answer - Ocular (10x) × objective lens.


Q: How long should proper handwashing take? -
correct answer - About 20 seconds.


Q: How many dyes are used in a simple stain? -
correct answer - One.


Q: What are conidia? - correct answer - Asexual
spores.

, Q: What are general-purpose media used for? -
correct answer - Growing many types of microbes.


Q: What are hyphae? - correct answer - Filament
structures.


Q: What are key steps in aseptic technique? -
correct answer - Flame loop, keep plates closed,
work near flame.


Q: What are sporangiospores? - correct answer -
Spores in a sac.


Q: What are the Gram stain steps in order? -
correct answer - Crystal violet → iodine → alcohol
→ safranin.


Q: What color are Gram-negative bacteria? -
correct answer - Pink.


Q: What color are Gram-positive bacteria? -
correct answer - Purple.
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