Pure culture technique
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® a method to dilute a large population of cells in a mixed culture so that
cells are separated or on an agar medium to form isolated colonies
order of dyes/chemicals for gram staining
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primary stain: crystal violet
mordant: gram's iodine
decolorizing agent: ethanol
counterstain: safranin
,Fluid thioglycolate medium (FTM
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o viscous liquid (semi-solid) that contains a reducing agent (sodium
thioglycolate) that will tie up oxygen to create an anaerobic environment in
the lower portion of the tube
o Screw cap tubes used to inhibit atmospheric O2 from diffusing into
medium
non septate hypae
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no cross walls
common bacterial shapes
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o Rod (bacilli)
o Spherical (cocci)
Spiral (helical or curved rods)
yeast are fungi that do not normally form ____________ whereas molds are mostly
composed of _____________
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, hyphae
hyphae
The purposes of HEAT FIXING
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· Bacteria on the slide will be killed by the heat
· 2. Cells will be "fixed" by the heat, causing them to adhere to the slide so
that the cells will not wash off when applying staining and rinsing solutions
where do they grow
Obligate aerobes
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only grow near the surface where there is O2
Characteristics of a good smear:
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smear is not too thick
cells are adhered to the slide via heat fixing
represents accurate size and shape of cells
, mechanical stage know
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moves specimen around on stage
100x magnification
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oil immersion
Mesophile
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temperatures between 20°C and 40°C, Serratia marcescens
media used in kirby bauer
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o Mueller-Hinton II agar
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® a method to dilute a large population of cells in a mixed culture so that
cells are separated or on an agar medium to form isolated colonies
order of dyes/chemicals for gram staining
Give this one a try later!
primary stain: crystal violet
mordant: gram's iodine
decolorizing agent: ethanol
counterstain: safranin
,Fluid thioglycolate medium (FTM
Give this one a try later!
o viscous liquid (semi-solid) that contains a reducing agent (sodium
thioglycolate) that will tie up oxygen to create an anaerobic environment in
the lower portion of the tube
o Screw cap tubes used to inhibit atmospheric O2 from diffusing into
medium
non septate hypae
Give this one a try later!
no cross walls
common bacterial shapes
Give this one a try later!
o Rod (bacilli)
o Spherical (cocci)
Spiral (helical or curved rods)
yeast are fungi that do not normally form ____________ whereas molds are mostly
composed of _____________
Give this one a try later!
, hyphae
hyphae
The purposes of HEAT FIXING
Give this one a try later!
· Bacteria on the slide will be killed by the heat
· 2. Cells will be "fixed" by the heat, causing them to adhere to the slide so
that the cells will not wash off when applying staining and rinsing solutions
where do they grow
Obligate aerobes
Give this one a try later!
only grow near the surface where there is O2
Characteristics of a good smear:
Give this one a try later!
smear is not too thick
cells are adhered to the slide via heat fixing
represents accurate size and shape of cells
, mechanical stage know
Give this one a try later!
moves specimen around on stage
100x magnification
Give this one a try later!
oil immersion
Mesophile
Give this one a try later!
temperatures between 20°C and 40°C, Serratia marcescens
media used in kirby bauer
Give this one a try later!
o Mueller-Hinton II agar
