MCB 2010 REVIEW EXAMS ALL QUESTIONS AND
ANSWERS SURE A+
✔✔141) An experiment began with 4 cells and ended with 128 cells. How many
generations did the
cells go through? - ✔✔D) 5
✔✔142) Three cells with generation times of 60 minutes are inoculated into a culture
medium. How many cells are there after 5 hours? - ✔✔C) 96
✔✔143) In Figure 6.3, which tube shows the expected growth pattern for a
microaerophile? - ✔✔E) e
✔✔144) In Figure 6.3, which tube shows the expected growth pattern for a facultative
anaerobe? - ✔✔B) b
✔✔145) In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary tract
of 10 percent of 1300 patients who underwent gastrointestinal endoscopic procedures.
After each use, endoscopes were washed with an automatic reprocessor that flushed
detergent and glutaraldehyde through the endoscopes, followed by a tap water rinse. P.
aeruginosa serotype 10 was not isolated from the detergent, glutaraldehyde, or tap
water. What was the source of the infections? - ✔✔B) a biofilm in the reprocessor
✔✔146) Assume you inoculated 100 cells, with a generation time of 20 minutes, into
100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml
of nutrient broth. After incubation for 4 hours, you can reasonably expect to have -
✔✔C) the same number of cells in both.
✔✔147) The source of nutrients in nutrient agar is - ✔✔C) peptone and beef extract.
, ✔✔148) Which enzyme catalyzes the reaction: O2- + O2- + 2H+ → H2O2 + O2? -
✔✔D) superoxide dismutase
✔✔149) Which enzyme catalyzes the reaction: 2H2O2 → 2H2O + O2? - ✔✔C)
peroxidase
✔✔150) Which enzyme catalyzes the reaction: H2O2 + 2H+ → 2H2O? - ✔✔A) catalase
✔✔The data in Table 6.1 indicate that S. aureus is a(n) - ✔✔C) facultative halophile.
✔✔152) Patients with indwelling catheters (long-term tubes inserted into body orifices
for drainage, such as through the urethra and into the urinary bladder) are susceptible
to infections because - ✔✔D) biofilms develop on catheters.
✔✔153) An isolated colony on a streak plate contains millions (or even billions) of
identical cells all arising from one initial cell. - ✔✔A) TRUE
✔✔154) Bacterial growth refers to an increase in the numbers of cells in a bacterial
culture. - ✔✔A) TRUE
✔✔155) Pure cultures can easily be obtained on streak plates, even if the desired
bacteria are present in very low concentrations in the initial culture broth. - ✔✔B)
FALSE
✔✔156) Agar is used as a solidifying agent in microbiological media since few bacteria
can degrade it. - ✔✔A) TRUE
✔✔157) Laboratory cultivation of obligate anaerobes requires reducing media or special
growth chambers filled with inert gases. - ✔✔A) TRUE
✔✔158) Most pathogenic bacteria are thermophiles. - ✔✔B) FALSE
✔✔159) In performing a ten-fold dilutions series from a sample containing 10,000
bacteria per milliliter, the fourth tube in the dilution series will have 10 cells per milliliter.
- ✔✔B) FALSE
✔✔160) Turbidity is an indirect measurement of bacterial growth that can be measured
using a spectrophotometer. - ✔✔A) TRUE
✔✔161) Filtration methods, followed by growth of the bacteria trapped on the filters in
growth media, are used to count bacteria present in very low concentrations, such as in
lakes and streams. - ✔✔A) TRUE
ANSWERS SURE A+
✔✔141) An experiment began with 4 cells and ended with 128 cells. How many
generations did the
cells go through? - ✔✔D) 5
✔✔142) Three cells with generation times of 60 minutes are inoculated into a culture
medium. How many cells are there after 5 hours? - ✔✔C) 96
✔✔143) In Figure 6.3, which tube shows the expected growth pattern for a
microaerophile? - ✔✔E) e
✔✔144) In Figure 6.3, which tube shows the expected growth pattern for a facultative
anaerobe? - ✔✔B) b
✔✔145) In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary tract
of 10 percent of 1300 patients who underwent gastrointestinal endoscopic procedures.
After each use, endoscopes were washed with an automatic reprocessor that flushed
detergent and glutaraldehyde through the endoscopes, followed by a tap water rinse. P.
aeruginosa serotype 10 was not isolated from the detergent, glutaraldehyde, or tap
water. What was the source of the infections? - ✔✔B) a biofilm in the reprocessor
✔✔146) Assume you inoculated 100 cells, with a generation time of 20 minutes, into
100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml
of nutrient broth. After incubation for 4 hours, you can reasonably expect to have -
✔✔C) the same number of cells in both.
✔✔147) The source of nutrients in nutrient agar is - ✔✔C) peptone and beef extract.
, ✔✔148) Which enzyme catalyzes the reaction: O2- + O2- + 2H+ → H2O2 + O2? -
✔✔D) superoxide dismutase
✔✔149) Which enzyme catalyzes the reaction: 2H2O2 → 2H2O + O2? - ✔✔C)
peroxidase
✔✔150) Which enzyme catalyzes the reaction: H2O2 + 2H+ → 2H2O? - ✔✔A) catalase
✔✔The data in Table 6.1 indicate that S. aureus is a(n) - ✔✔C) facultative halophile.
✔✔152) Patients with indwelling catheters (long-term tubes inserted into body orifices
for drainage, such as through the urethra and into the urinary bladder) are susceptible
to infections because - ✔✔D) biofilms develop on catheters.
✔✔153) An isolated colony on a streak plate contains millions (or even billions) of
identical cells all arising from one initial cell. - ✔✔A) TRUE
✔✔154) Bacterial growth refers to an increase in the numbers of cells in a bacterial
culture. - ✔✔A) TRUE
✔✔155) Pure cultures can easily be obtained on streak plates, even if the desired
bacteria are present in very low concentrations in the initial culture broth. - ✔✔B)
FALSE
✔✔156) Agar is used as a solidifying agent in microbiological media since few bacteria
can degrade it. - ✔✔A) TRUE
✔✔157) Laboratory cultivation of obligate anaerobes requires reducing media or special
growth chambers filled with inert gases. - ✔✔A) TRUE
✔✔158) Most pathogenic bacteria are thermophiles. - ✔✔B) FALSE
✔✔159) In performing a ten-fold dilutions series from a sample containing 10,000
bacteria per milliliter, the fourth tube in the dilution series will have 10 cells per milliliter.
- ✔✔B) FALSE
✔✔160) Turbidity is an indirect measurement of bacterial growth that can be measured
using a spectrophotometer. - ✔✔A) TRUE
✔✔161) Filtration methods, followed by growth of the bacteria trapped on the filters in
growth media, are used to count bacteria present in very low concentrations, such as in
lakes and streams. - ✔✔A) TRUE
