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BIO 251 Exam 2 Questions & Answers | 2026/2027 Biology Exam Review | Practice Test with Detailed Rationales & Verified Solutions | Graded A+

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BIO 251 Exam 2 is a comprehensive exam preparation resource designed to help biology students review important course concepts and prepare effectively for their second examination. Featuring exam-focused practice questions with 100% correct and verified answers, this study guide reinforces key biological principles, terminology, processes, and concepts commonly tested in BIO 251 coursework. Ideal for quizzes, midterms, and exam revision, it supports active recall, efficient studying, and improved confidence for academic success in biology.

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Institution
Biol 251
Course
Biol 251

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Bio 251 - Exam 2
(2026-2027 Edition)

Questions with 100% Correct
Answers and Rationales | Latest
Update | Grade A+

,the study of how the heredity material (DNA and RNA) is genetics
transferred.



all genetic material in a cell genome




what is the sub division of genome - chromosome
- plasmid



what is the sub division of chromosomes genes (coding region)
non-coding region



what composes the DNA back bone sugar
phosphate



which are the 4 nucleotides in DNA Adenine
Thymine
Cytosine
Guanine

Uracil (only in RNA)


what is the difference between bacterial and Eukaryota Most bacteria have only one circular chromosome, whereas eukaryotes tend to
chromosomes have multiple linear chromosomes



Why is DNA semi-conservative? When DNA divides, each copy receives one half of the original DNA and one half
new DNA.



enzyme(s) that uncoiled the DNA topoisomerase and gyrase, and begins at a region called the origin of replication




enzyme that unwinds and begins separating the DNA helicase
strands in both directions



enzyme that stabilize the single stranded DNA single strand binding proteins




what is required for the addition of nucleotides in DNA a free 3' hydroxyl group
replication?



enzyme that adds an RNA primer to the strand being primase
replicated



enzyme that makes sure the proper nucleotide is bound, DNA polymerase
and connects it to the growing DNA strand



DNA lagging strand - DNA is synthesized in pieces
- multiple RNA primers are added
- assembled together by the enzymes DNA -polymerase and ligase

, 3 main process of DNA replication - initiation
- elongation
- termination


Central Dogma DNA -> RNA -> Protein

The process by which DNA is used to encode proteins


what some of the characteristics of transcription/RNA - Only specific regions are copies
- RNA strands are produced, which do not bind with the DNA (exist as single
strands)
- No proofreading


what are the 5 steps in transcription Step 1: RNA polymerase binds to the promoter (a regulatory region directly
upstream of the gene) and initiates DNA unraveling

Step 2 : RNA polymerase facilitates the assembly of free ribonucleotides into
chains of RNA, using the DNA as a template

Step 3: The RNA polymerase continues along the gene adding to the RNA chain,
which does not permanently pair with the DNA, but exists as a single strand

Step 4: RNA synthesis continues until the RNA polymerase encounters a specific
region of DNA known as a terminator

Step 5: Once the terminator is reached, the RNA polymerase stalls and a rho
termination protein facilitates the RNA polymerase and RNA strand to
disassociate from the DNA
The DNA is re-wound and the RNA is free to go on to the next step


name for groups of 3 base pairs of the mRNA codons




how many codons are non-coding or stop codons? 3 are non-coding or stop codons
only 61 of the 64 encode amino acids



which molecule can bind both RNA and amino acids T-RNA




group of three bases on a tRNA molecule that are Anticodon
complementary to an mRNA codon



t-RNA is considered to be "charged" when an amino acid is bound to t-RNA




how many different t-RNA there are 64, one for each codon




the structures where protein synthesis (translation) Ribosomes
occurs



which are the 2 microbial ribosome subunits the 30S and 50S, which come together to form a functional 70S ribosome




what does the "S" in ribosomal subunits stands for Svedberg units, which measures the rate of sedimentation during centrifugation

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