ASU BIO 340 EXAM 3 2026 EDITION
COMPREHENSIVE PRACTICE QUESTIONS
AND ANSWERS COMPLETE GENERAL
GENETICS EXAM PREPARATION
RESOURCE GENE REGULATION,
POPULATION GENETICS, MOLECULAR
GENETICS, EVOLUTION AND DETAILED
STUDY GUIDE
What is the sequence of the template DNA used in the sequencing reactions
below? A. 5' TAGTCTCGATATCGTCACG 3' B. 5'
ATCAGAGCTATAGCAGTGC 3' C. 3' TAGTCTCGATATCGTCACG 5' D. 3'
ATCAGAGCTATAGCAGTGC 5' - CORRECT ANSWER -D Explanation: We
are looking for the reverse complement of the sequenced strand. A and C
show the sequenced strand, and B the complement, but not from 3' to 5
Which of the following restriction enzyme(s) is/are a 6-cutter? A. EcoRI,
SpeI, SphI, StuI, KpnI B. Taqi and Sau3AI C. EcoRI, SpeI, SphI, KpnI, TaqI,
Sau3A1 D. EcoRI, SpeI, TaqI, Sau3A1 E. SphI and KpnI - CORRECT
ANSWER -A Explanation: 6-cutter = six nucleotides wide
Which of the following restriction enzyme(s) leave(s) a 3' overhang? A.
EcoRI, SpeI, SphI, StuI, KpnI B. Taqi and Sau3AI C. EcoRI, SpeI, SphI,
KpnI, TaqI, Sau3A1 D. EcoRI, SpeI, TaqI, Sau3A1 E. SphI and KpnI -
CORRECT ANSWER -E Explanation: When the text says 3' overhang, the
, overhang ends with an exposed 3' end. This means that the longer sequence
is on the 5' strand.
Given the linear DNA molecule shown below, how many fragments would
you expect if it was cut with BamHI and BcII?
A. 1 B. 2 C. 3 D. 4 E. 5 - CORRECT ANSWER -C Explanation: BamHI and BclI
will cut the double-strand only once each, having three pieces of double-
stranded DNA as a product.
Can the sticky ends produced by BamHI and BcII be ligated together?
A. Yes B. No - CORRECT ANSWER -A Explanation: The overhangs left by
each enzyme are complementary to each other
Can the resulting ligated sequences be cut by BamHI A. Yes B. No -
CORRECT ANSWER -B Explanation: The resulting ligated sequence no
longer has the recognition sequence for BamHI. So the enzyme can no
longer cut it
Molecular cloning involves cutting a gene from its normal location,
inserting it into a circular piece of DNA, and then transferring the circle of
DNA into a bacterial or yeast cell. Which of the following is used to cut the
gene from its normal location? A. Restriction enzyme B. Plasmid C.
Bacteriophage D. Vector E. Ligase - CORRECT ANSWER -A. restriction
enzyme
Molecular cloning involves cutting a gene from its normal location,
inserting it into a circular piece of DNA, and then transferring the circle of
DNA into a bacterial or yeast cell. Which of the following describes the
circular DNA from a bacterial cell? A. Restriction enzyme B. Plasmid C.
COMPREHENSIVE PRACTICE QUESTIONS
AND ANSWERS COMPLETE GENERAL
GENETICS EXAM PREPARATION
RESOURCE GENE REGULATION,
POPULATION GENETICS, MOLECULAR
GENETICS, EVOLUTION AND DETAILED
STUDY GUIDE
What is the sequence of the template DNA used in the sequencing reactions
below? A. 5' TAGTCTCGATATCGTCACG 3' B. 5'
ATCAGAGCTATAGCAGTGC 3' C. 3' TAGTCTCGATATCGTCACG 5' D. 3'
ATCAGAGCTATAGCAGTGC 5' - CORRECT ANSWER -D Explanation: We
are looking for the reverse complement of the sequenced strand. A and C
show the sequenced strand, and B the complement, but not from 3' to 5
Which of the following restriction enzyme(s) is/are a 6-cutter? A. EcoRI,
SpeI, SphI, StuI, KpnI B. Taqi and Sau3AI C. EcoRI, SpeI, SphI, KpnI, TaqI,
Sau3A1 D. EcoRI, SpeI, TaqI, Sau3A1 E. SphI and KpnI - CORRECT
ANSWER -A Explanation: 6-cutter = six nucleotides wide
Which of the following restriction enzyme(s) leave(s) a 3' overhang? A.
EcoRI, SpeI, SphI, StuI, KpnI B. Taqi and Sau3AI C. EcoRI, SpeI, SphI,
KpnI, TaqI, Sau3A1 D. EcoRI, SpeI, TaqI, Sau3A1 E. SphI and KpnI -
CORRECT ANSWER -E Explanation: When the text says 3' overhang, the
, overhang ends with an exposed 3' end. This means that the longer sequence
is on the 5' strand.
Given the linear DNA molecule shown below, how many fragments would
you expect if it was cut with BamHI and BcII?
A. 1 B. 2 C. 3 D. 4 E. 5 - CORRECT ANSWER -C Explanation: BamHI and BclI
will cut the double-strand only once each, having three pieces of double-
stranded DNA as a product.
Can the sticky ends produced by BamHI and BcII be ligated together?
A. Yes B. No - CORRECT ANSWER -A Explanation: The overhangs left by
each enzyme are complementary to each other
Can the resulting ligated sequences be cut by BamHI A. Yes B. No -
CORRECT ANSWER -B Explanation: The resulting ligated sequence no
longer has the recognition sequence for BamHI. So the enzyme can no
longer cut it
Molecular cloning involves cutting a gene from its normal location,
inserting it into a circular piece of DNA, and then transferring the circle of
DNA into a bacterial or yeast cell. Which of the following is used to cut the
gene from its normal location? A. Restriction enzyme B. Plasmid C.
Bacteriophage D. Vector E. Ligase - CORRECT ANSWER -A. restriction
enzyme
Molecular cloning involves cutting a gene from its normal location,
inserting it into a circular piece of DNA, and then transferring the circle of
DNA into a bacterial or yeast cell. Which of the following describes the
circular DNA from a bacterial cell? A. Restriction enzyme B. Plasmid C.
