(100 QUESTIONS AND CORRECT
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Introduction
This structured NHCO Phlebotomy Exam format for 2026–2027 provides the complete
layout for generating high-quality exam-style questions with correct answers and
rationales. It emphasizes proper venipuncture and capillary puncture techniques, specimen
handling, infection control, and patient safety principles critical to professional phlebotomy
practice and successful national certification.
1. What is the most critical step in performing any phlebotomy procedure?
A. Applying the tourniquet
B. Selecting the correct needle size
C. Patient identification
D. Checking for vein patency
Rationale: Proper identification using at least two unique identifiers (e.g., name and DOB)
is the foundation of patient safety and prevents diagnostic errors.
2. Which of the following is the preferred site for venipuncture in adults?
A. Basilic vein
B. Median cubital vein
C. Cephalic vein
D. Metacarpal vein
Rationale: The median cubital is the first choice because it is typically larger, more
anchored, and carries the lowest risk of nerve damage.
,3. When performing a capillary puncture on an infant's heel, what is the maximum
depth allowed?
A. 1.0 mm
B. 2.0 mm
C. 3.0 mm
D. 5.0 mm
Rationale: Depths exceeding 2.0 mm risk hitting the calcaneus (heel bone), which can cause
osteomyelitis or osteochondritis.
4. According to the CLSI Order of Draw, which tube should be collected first?
A. Lavender (EDTA)
B. Green (Heparin)
C. Light Blue (Sodium Citrate)
D. Blood Culture tubes
Rationale: Blood cultures are collected first to maintain sterility and prevent
contamination of the specimen.
5. The purpose of the 'additive' in a Light Blue top tube is to:
A. Promote clotting
B. Preserve glucose
C. Inhibit thrombin
D. Bind calcium to prevent clotting
Rationale: Sodium citrate is an anticoagulant that binds calcium; it is essential for
coagulation studies.
6. Which antiseptic is most commonly used for routine venipuncture?
A. 70% Isopropyl alcohol
B. Povidone-iodine
, C. Chlorhexidine gluconate
D. Soap and water
Rationale: Isopropyl alcohol is the standard for routine cleansing. Povidone-iodine is
typically reserved for blood cultures or arterial punctures.
7. A tourniquet should remain on the patient's arm for no longer than:
A. 30 seconds
B. 1 minute
C. 2 minutes
D. 5 minutes
Rationale: Leaving a tourniquet on too long causes hemoconcentration, altering the
concentration of cells and analytes in the blood sample.
8. What is the correct angle for needle insertion during a standard venipuncture?
A. 5-10 degrees
B. 15-30 degrees
C. 45 degrees
D. 90 degrees
Rationale: This angle range allows for optimal entry into the vein without passing through
it or causing excessive trauma.
9. Infection control practices that treat all body fluids as potentially infectious are
called:
A. Basic precautions
B. Universal/Standard precautions
C. Isolation protocols
D. Contact precautions