Microbiology Lab Exam 1 with correct answers 100% 2026

Microbiology Lab Exam 1 with correct answers 100% 2026 When should hands be washed? - Correct Answer Wash your hands thoroughly with soap and water after handling living microbes and before leaving the laboratory each day. Also, wash your hands after removing gloves. How do you clean your lab bench? - Correct Answer Wipe the desktop with a disinfectant (e.g., Coverage or 10% chlorine bleach) before and after each lab period. Allow disinfectant to evaporate; do not wipe dry Why must Bunsen burners and incinerators be treated with caution even after you have finished using them? - Correct Answer It is a fire & safety hazard as well as an additional source of unnecessary heat in the room When should you sterilize your loop? - Correct Answer Before and after use through Bunsen burner if BSL-1 or in enclosed electric incinerator if BSL-2 (also, sterile disposable loops/wooden sticks) Describe 2 specialized waste containers that can be found in lab: - Correct Answer 1) sharps/broken glass container: disposal of broken glass or any other item that could puncture an autoclave bag 2)autoclave container: disposal of plate cultures and other contaminated non-sharp disposable items Describe appropriate attire that should be worn in lab: - Correct Answer 1) Wear eye protection whenever heating chemicals 2)Wear disposable gloves while staining microbes and handling blood products 3) Lab coat 4) close-toed toes Why must electronic devices and other personal items be put away before entering the lab room and not brought out before fully exiting the room? - Correct Answer Contamination of these items can be a health hazard as well as expensive. What is the protocol if infectious material is spilled? - Correct Answer Cover any culture spills with paper towels. Soak the towels immediately with disinfectant and allow them to stand for 20 minutes. Report spill to the instructor. When you are finished, place towels in container for autoclaving Where should gloves be disposed of? Why? - Correct Answer in container designated for autoclaving/biohazardous waste bin to reduce risk of contamination The lysis of red blood cells in blood agar is indicative of the presence of which enzymes? - Correct Answer streptolysin O and S When looking at a blood agar plate with bacterial growth, beta is complete lysis of the red blood cells, alpha is partial lysis, and gamma is no lysis. - Correct Answer True An organism that obtains its nutrients from dead organic matter is a(n) __________. - Correct Answer saprophyte A reservoir is - Correct Answer a source of microbial contamination. Commensalism is best described as a(n) - Correct Answer relationship between two organisms where only one member benefits and the other is unharmed.