Microbiology Lab Exam 1 Note Cards with correct answers 100% 2026

when viewing a smear with the oil immersion lens the final magnification is - Correct Answer 1000x;10x ocular x 100X objective what is the purpose of heat fixing - Correct Answer kill the bacteria, adhere to glass anddenature the proteins of bacteria name oneof the stains or regents used in the gram stain procedure - Correct Answer gram crystal violet, gram iodine, gram sfaranin, 95% ethanol why are aseptic techniques used in the lab? - Correct Answer to ensure that we do not contaminate what we are working with as as to ensue that we do not acciently inoculate ouselves. example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before and after obtaining the sample. the gram reaction is due to differences in the structure of wat part of the microbe - Correct Answer its cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer compared to GN bacteria. Name 2 inoculating tools you will be using in this lab - Correct Answer loop, needle what color is a gram positive microbe - Correct Answer purple waht is the function of the ethanol in the gram stain procedure - Correct Answer decolorize name one of the strains in the acid fast stain - Correct Answer cold kinyoun, carbol fuschsin, methylen blue name one of the stains in the endospore stain - Correct Answer malachite green, safranin what is the purpose of the acid-fast stain - Correct Answer the purpose of the acid-fast stain is to differentiate between the myobactriace family of bacteria from all other bacteria what color does na acid-fast microbe stain with acd-fast stain - Correct Answer red what is the function of agarin the media - Correct Answer agar allows media to remain solid at room temperature what is the difference between broth and agar based media - Correct Answer broth is liquid media at room temperature, agar media is solid at room temp. what is the funciton of media - Correct Answer media is used to provide nutrients to a microbe as well as a growth medium what is the name of the plate inoculation technique - Correct Answer quadrant steak plate Why must the media we are making today be sterilized prior to us? - Correct Answer the meda must be sterilized to remove any bacterial contaminant that may be introduced when the media is made how do you know yo have dont a successful streak plate? - Correct Answer youve ended up with isolated colonies what do you think will happen if you inoculated an obligate anerobe onto a TSA plate and incubated the incubator we have been using? - Correct Answer the obligate anerobe would not grow because it doesn't have the ability to produce th necessary enzymes to dtoxify toxic oxygen species