MIMG Exam III Questions & Answers
(Grade A+)
What percentage of microbes have been cultured in the laboratory?
-
correct answer ✅1%
A traditional technique used to quantify specific types of microbes
in natural samples, enrichment cultures, and food or water samples
is known as the ________ technique. -
correct answer ✅Most Probable Number
_______ cultures are pure cultures, consisting of a single species. -
correct answer ✅Axenic
The use of modern molecular techniques has provided a
mechanism for growing some of the microorganisms previously
referred to as unculturable. -
correct answer ✅TRUE
The discrepancy between the number of microbial cells observed
by microscopic examination and the number of colonies that can be
cultivated from the same natural sample may be due to _____ -
correct answer ✅nonviable organisms and incorrect culture
conditions
,MIMG Exam III Questions & Answers
(Grade A+)
________ culture techniques are based on the expansion of the
microenvironment to allow abundant growth of a microorganism
formerly restricted to a small ecological niche -
correct answer ✅Enrichment
Optical tweezers can be used to -
correct answer ✅study nucleic acids recovered from individual
cells
Recent evidence suggests that most microbial communities have
many individuals representing
at least _____ taxa -
correct answer ✅10,000,000
Determining the functional nature of a microbial community by
analysis of bulk DNA samples extracted from environmental
samples may be misleading for all of the following reasons EXCEPT
_____
A) DNA is of little use in identifying unknown microbes
B) some of the DNA recovered may be from nonliving organisms C)
some of the DNA may be from entities such as spores
,MIMG Exam III Questions & Answers
(Grade A+)
D) the DNA obtained can depend on the extraction method used -
correct answer ✅A) DNA is of little use in identifying unknown
microbes
When PCR amplification of SSU (small subunit ribosomal RNA)
yields a population of different PCR products of about the same
molecular weight, these fragments can be separated for further
analysis by _____ -
correct answer ✅denaturing gradient gel electrophoresis
When doing SSU (small subunit ribosomal RNA) of a mixed
population of microbes from an environmental sample, specific
____ are identified -
correct answer ✅phylotypes
It is generally agreed that SSU (small subunit ribosomal RNA)
analysis is a technique that is well suited to identifying species
present in a given community -
correct answer ✅TRUE
Which of the following is microscopy in which the microscope is
fitted with light filters that enable the excitation of the specimen
, MIMG Exam III Questions & Answers
(Grade A+)
with a specific wavelength? -
correct answer ✅Epifluorescence microscopy
When fluorescently labeled oligonucleotides that are known to be
specific to the microbe of interest are used to label natural samples,
the technique is known as ____ -
correct answer ✅Fluorescent In Situ Hybridization
The fact that certain nucleotide templates are more readily
amplified than others is termed ____ -
correct answer ✅PCR bias
The level of specific mRNA, and therefore the expression of a given
gene in a particular ecosystem, can be evaluated in the
environment using in situ reverse transcriptase (ISRT)-FISH -
correct answer ✅TRUE
The goal of ______ is to identify each protein present in a given
microenvironment at the time of sampling. -
correct answer ✅Metaproteomics
(Grade A+)
What percentage of microbes have been cultured in the laboratory?
-
correct answer ✅1%
A traditional technique used to quantify specific types of microbes
in natural samples, enrichment cultures, and food or water samples
is known as the ________ technique. -
correct answer ✅Most Probable Number
_______ cultures are pure cultures, consisting of a single species. -
correct answer ✅Axenic
The use of modern molecular techniques has provided a
mechanism for growing some of the microorganisms previously
referred to as unculturable. -
correct answer ✅TRUE
The discrepancy between the number of microbial cells observed
by microscopic examination and the number of colonies that can be
cultivated from the same natural sample may be due to _____ -
correct answer ✅nonviable organisms and incorrect culture
conditions
,MIMG Exam III Questions & Answers
(Grade A+)
________ culture techniques are based on the expansion of the
microenvironment to allow abundant growth of a microorganism
formerly restricted to a small ecological niche -
correct answer ✅Enrichment
Optical tweezers can be used to -
correct answer ✅study nucleic acids recovered from individual
cells
Recent evidence suggests that most microbial communities have
many individuals representing
at least _____ taxa -
correct answer ✅10,000,000
Determining the functional nature of a microbial community by
analysis of bulk DNA samples extracted from environmental
samples may be misleading for all of the following reasons EXCEPT
_____
A) DNA is of little use in identifying unknown microbes
B) some of the DNA recovered may be from nonliving organisms C)
some of the DNA may be from entities such as spores
,MIMG Exam III Questions & Answers
(Grade A+)
D) the DNA obtained can depend on the extraction method used -
correct answer ✅A) DNA is of little use in identifying unknown
microbes
When PCR amplification of SSU (small subunit ribosomal RNA)
yields a population of different PCR products of about the same
molecular weight, these fragments can be separated for further
analysis by _____ -
correct answer ✅denaturing gradient gel electrophoresis
When doing SSU (small subunit ribosomal RNA) of a mixed
population of microbes from an environmental sample, specific
____ are identified -
correct answer ✅phylotypes
It is generally agreed that SSU (small subunit ribosomal RNA)
analysis is a technique that is well suited to identifying species
present in a given community -
correct answer ✅TRUE
Which of the following is microscopy in which the microscope is
fitted with light filters that enable the excitation of the specimen
, MIMG Exam III Questions & Answers
(Grade A+)
with a specific wavelength? -
correct answer ✅Epifluorescence microscopy
When fluorescently labeled oligonucleotides that are known to be
specific to the microbe of interest are used to label natural samples,
the technique is known as ____ -
correct answer ✅Fluorescent In Situ Hybridization
The fact that certain nucleotide templates are more readily
amplified than others is termed ____ -
correct answer ✅PCR bias
The level of specific mRNA, and therefore the expression of a given
gene in a particular ecosystem, can be evaluated in the
environment using in situ reverse transcriptase (ISRT)-FISH -
correct answer ✅TRUE
The goal of ______ is to identify each protein present in a given
microenvironment at the time of sampling. -
correct answer ✅Metaproteomics
