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NHA CPT Practice Test – Study Guide Questions and Verified Answers – National Healthcareer Association (NHA) Certified Phlebotomy Technician (CPT) – 2026/2027 Certification Exam Review Guide

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This document contains study guide questions and verified answers designed to help candidates prepare for the National Healthcareer Association (NHA) Certified Phlebotomy Technician (CPT) certification examination. It covers essential topics including venipuncture techniques, capillary collection, specimen handling and processing, infection prevention and control, patient identification, laboratory safety, medical terminology, quality assurance, and professional responsibilities. The material is organized in a question-and-answer format to reinforce key phlebotomy concepts, support effective exam preparation, and improve certification readiness. It serves as a comprehensive study resource for students and healthcare professionals preparing for the 2026/2027 NHA Certified Phlebotomy Technician (CPT) certification examination.

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1. 1st in order of draw: Yellow Tubes
2. Whichtubeis sterile?:YellowToppedTubes
3. Additive for Yellow Tubes: SPS, ACD
4. Common Test for Yellow Tubes: Blood Cultures
5. Department: Yellow Tubes: Microbiology
6. Inversion: Yellow Tubes: 8-10 times
7. Specimen: Yellow Tubes: Whole Blood
8. 2nd in order of draw: Light Blue Tubes
9. Additive: Light Blue Tubes: SodiumCitrate
10. CommonTest: Light Blue Tubes: PT, APTT, CoagulationStudies
11. Department:Light Blue Tubes: CoagulationStudies
12. Inversion: Light Blue Tubes: 3-4 times
13. Specimen: Light Blue Tubes: Plasma
14. 3rd in order of draw: Red Tubes
15. Additive: Red Tubes: None
16. CommonTest: Red Tubes: Immunology,Serology,BloodBank
17. Department: Red Tubes: Chemistry
18. Inversion: Red Tubes: 5 times
19. Specimen: Red Tubes: Serum
20. 4th in order of draw: Tiger (speckled)ToppedTubes
21. Additive: Tiger (speckled) Topped Tubes: Clot Activator
22. Test: Tiger (speckled) Topped Tubes: CMP,Endocrine,Immunology,Serology
23. Department: Tiger (speckled) Topped Tubes: Chemistry
24. Inversion: Tiger (speckled) Topped Tubes: 5 times


,25. Specimen: Tiger (speckled) Topped Tubes: Serum
26. 5th in order of draw: Gold Tubes
27. Additive: Gold Tubes: Thrombin (Causes bloodto clot in <5 minutes)
28. Common Test: Gold Tubes: STAT
29. Department: Gold Tubes: Chemistry
30. Inversion: Gold Tubes: 5-6 times
31. Specimen: Gold Tubes: Serum
32. 6th in order of draw: Green Tubes






, 33. Additive: Green Tubes: Heparin
34. CommonTest: GreenTubes: STAT ChemistryTest, AmmoniaElectrolytes,ABGs
35. Department:Green Tubes: Chemistry or MolecularLab
36. Inversion: Green Tubes: 8-10 Times
37. Specimen: Green Tubes: Plasma
38. 7th in order of draw: Lavender/PurpleTubes
39. Additive: Lavender Tubes: EDTA
40. CommonTest: LavenderTubes: CBC, ESR, Hemaglobin
41. Department: Lavender Tubes: Hematology
42. Inversion: Lavender Tubes: 8-10 times
43. Specimen: Lavender Tubes: Whole Blood
44. 8th in order of draw: Gray Tubes
45. Additive:Gray Tubes: SodiumFluorideand PotassiumOẋalate
46. CommonTest: Gray Tubes: Lactic Acid, BloodAlcoholLevels, FBS, GlucoseTolerance
47. Department: Gray Tubes: Chemistry
48. Inversion: Grey Tubes: 8-10 times
49. Specimen: Grey Tubes: Plasma
50. Additive: Tan Tubes: K2, EDTA
51. Test: Tan Tubes: Lead
52. Department: Tan Tubes: Chemistry
53. Inversion: Tan Tubes: Chemistry
54. Specimen: Tan Tubes: Plasma
55. Additive: Royal Blue: Heparin, EDTA, None
56. CommonTest: Royal Blue: Toẋiology,TraceMetal
57. Department: Royal Blue: Chemistry
58. Inversion: Royal Blue: 8-10 Times
59. Specimen:Royal Blue: Plasma or Serum
60. Additive:Black Tube: SodiumCitrate

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