Microbiology LAB Exam #1 Questions With Complete
Solutions
= How many decimel places, to the right of base Correct
Answers Six places --- .000 000
6.02x 10 = moving the decimel over how many spaces? Correct
Answers 23
An example of a producer is what? Correct Answers Algae are
producers that are found in a pond.
At what temperature, pressure, and time was required to sterilize
our TSA medium using the autoclave? Correct Answers The
autoclave will reach 121˚C at 15 pounds of pressure. 15 minutes
is required to sterilize a normal load (however if larger amounts
of liquid are being sterilized, more time may be required.)
Compare and contrast these three techniques by pattern of
growth on the surface Correct Answers Streak plates require
dilution by flaming the loop after streaking each quadrant and
only taking 3 streaks from the previous quadrant into the new
quadrant. By doing this, each quadrant is more dilute than the
previous quadrant. The 4th quadrant should always be dilute
enough to contain isolated colonies.
Pour plates have isolated colonies of bacteria evenly spread
throughout the medium (not just on the surface, but all
throughout the agar.). In the Pour Plate procedure, the bacteria is
diluted in sterile "water blanks" prior to plating the bacteria and
pouring agar over the bacteria. The bacteria sample that is dilute
,enough will produce isolated colonies throughout the medium
(rather than just on the surface.)
Spread Plate procedure is similar, to the pour plate in that the
bacteria is diluted prior to inoculation on the plate. The bacteria
sample is placed on an agar plate and then spread evenly across
the surface of the agar (rather than throughout the medium).
Compare and contrast these three techniques by where bacteria
grow in the media Correct Answers Streak Plate - bacteria
grow only on the surface
Pour Plate - bacteria grow throughout the media
Spread Plate - bacteria grow only on the surface
Convert Celsius to Fahrenheit Correct Answers F = (9xC)/5
+32
Convert Fahrenheit to Celsius Correct Answers C = 5/9 (F -
32)
define and identify the following agar slant growth patterns -
Arborescent- Correct Answers branched growth from the
original streak
define and identify the following agar slant growth patterns -
Beaded Correct Answers Isolated colonies toward the top of
the slant
define and identify the following agar slant growth patterns -
Echinulate Correct Answers pointed (spiny) edge of growth
, define and identify the following agar slant growth patterns -
Filiform Correct Answers Evenly distributed on slant (only
where streaked).
define and identify the following agar slant growth patterns -
Rhizoid Correct Answers Root like distribution of growth
(larger branches coming from the streak).
define and identify the following agar slant growth patterns -
Spreading Correct Answers Growth has spread out beyond the
original streak
define and identify the following broth culture growth patterns -
Flocculant Correct Answers clumps of growth throughout
define and identify the following broth culture growth patterns -
Pellicle Correct Answers A film of growth at the top of the
broth
define and identify the following broth culture growth patterns -
Sediment Correct Answers Precipitate that falls to the bottom
of the tube
define and identify the following broth culture growth patterns -
Turbidity- Correct Answers Growth evenly distributed
throughout.
Define the term sterilization Correct Answers Absence of all
microorganisms (spore-forming and viruses). Mcrobes are no
longer able to reproduce
Solutions
= How many decimel places, to the right of base Correct
Answers Six places --- .000 000
6.02x 10 = moving the decimel over how many spaces? Correct
Answers 23
An example of a producer is what? Correct Answers Algae are
producers that are found in a pond.
At what temperature, pressure, and time was required to sterilize
our TSA medium using the autoclave? Correct Answers The
autoclave will reach 121˚C at 15 pounds of pressure. 15 minutes
is required to sterilize a normal load (however if larger amounts
of liquid are being sterilized, more time may be required.)
Compare and contrast these three techniques by pattern of
growth on the surface Correct Answers Streak plates require
dilution by flaming the loop after streaking each quadrant and
only taking 3 streaks from the previous quadrant into the new
quadrant. By doing this, each quadrant is more dilute than the
previous quadrant. The 4th quadrant should always be dilute
enough to contain isolated colonies.
Pour plates have isolated colonies of bacteria evenly spread
throughout the medium (not just on the surface, but all
throughout the agar.). In the Pour Plate procedure, the bacteria is
diluted in sterile "water blanks" prior to plating the bacteria and
pouring agar over the bacteria. The bacteria sample that is dilute
,enough will produce isolated colonies throughout the medium
(rather than just on the surface.)
Spread Plate procedure is similar, to the pour plate in that the
bacteria is diluted prior to inoculation on the plate. The bacteria
sample is placed on an agar plate and then spread evenly across
the surface of the agar (rather than throughout the medium).
Compare and contrast these three techniques by where bacteria
grow in the media Correct Answers Streak Plate - bacteria
grow only on the surface
Pour Plate - bacteria grow throughout the media
Spread Plate - bacteria grow only on the surface
Convert Celsius to Fahrenheit Correct Answers F = (9xC)/5
+32
Convert Fahrenheit to Celsius Correct Answers C = 5/9 (F -
32)
define and identify the following agar slant growth patterns -
Arborescent- Correct Answers branched growth from the
original streak
define and identify the following agar slant growth patterns -
Beaded Correct Answers Isolated colonies toward the top of
the slant
define and identify the following agar slant growth patterns -
Echinulate Correct Answers pointed (spiny) edge of growth
, define and identify the following agar slant growth patterns -
Filiform Correct Answers Evenly distributed on slant (only
where streaked).
define and identify the following agar slant growth patterns -
Rhizoid Correct Answers Root like distribution of growth
(larger branches coming from the streak).
define and identify the following agar slant growth patterns -
Spreading Correct Answers Growth has spread out beyond the
original streak
define and identify the following broth culture growth patterns -
Flocculant Correct Answers clumps of growth throughout
define and identify the following broth culture growth patterns -
Pellicle Correct Answers A film of growth at the top of the
broth
define and identify the following broth culture growth patterns -
Sediment Correct Answers Precipitate that falls to the bottom
of the tube
define and identify the following broth culture growth patterns -
Turbidity- Correct Answers Growth evenly distributed
throughout.
Define the term sterilization Correct Answers Absence of all
microorganisms (spore-forming and viruses). Mcrobes are no
longer able to reproduce
