Biol 231 Exam 1 Questions With Complete Solutions
1/V = 1/Vmax + (KM/Vmax)(1/[S])match the following with
the correct terms.
-Which expression equals the slope of the line
-which expression equals the y-intercept of the line Correct
Answers KM/Vmax
1/Vmax
As we have seen, you can analyze the kinetic properties of a
Michaelis enzyme by measuring its velocity of catalysis at a
range of substrate concentrations and plotting [S] vs V, or 1/[S]
vs 1/V. You can also analyze the properties of an enzyme
inhibitor by carrying out the same experiment and graphic
analysis in the presence of the inhibitor. If you do this for a
noncompetitive inhibitor, what difference will you see when you
compare the enzyme kinetics to an uninhibited control?
Vmax and KM both remain the same with a non-competitive
inhibitor.
Vmax will decrease and KM will increase.
Vmax and KM will both decrease.
Vmax will be unchanged but KM will increase.
Vmax will decrease but KM will be unchanged. Correct
Answers Vmax will decrease but KM will be unchanged.
Assume that a spherical cell, with a diameter of 20 μm, has
spherical mitochondria, each with a diameter of 0.2 μm. If
mitochondria occupy 10% of the volume of the cell, how many
mitochondria are there? Correct Answers 100,000
, Assuming you have 10mL of a solution at pH 5 that is not
buffered, how much water would you need to add to the solution
in order to raise the pH to 6? Render your answer in mL
(milliliters). Correct Answers 90
Cells can take up molecules from the outside world by
endocytosis, through membrane channels, or by simple
diffusion. But regardless of the avenue, the rate at which the cell
can acquire material from the outside world is limited by the
total surface area of the cell. On the other hand, the amount of
material that they need to take up is proportional to the cell
volume - i.e., how much biomass is in the cell. Some cells are
spherical. Correct Answers 0.3
Cells in the body of a metazoan organism like yourself have
many shapes and sizes that are suited to their particular
functions. Consider a muscle cell, which has the shape of a long
cylinder. Assume a cell in one of the tiny dorsal interosseous
muscles of your hand is 14 mm long, with a radius of 12 μm.
What is its surface-to-volume ratio in μm-1? Correct Answers
0.1668
Consider the reaction:
Glucose + Pi ⇔ Glucose-6-phosphate + H20 Assume ΔGo =
+12.8 kJ/mol
What is the equilibrium constant, Keq, for this reaction? Correct
Answers 0.0056
Consider this equation:
Glucose + Pi ⇔ Glucose-6-phosphate + H20 ΔGo = +12.5
kJ/mol
1/V = 1/Vmax + (KM/Vmax)(1/[S])match the following with
the correct terms.
-Which expression equals the slope of the line
-which expression equals the y-intercept of the line Correct
Answers KM/Vmax
1/Vmax
As we have seen, you can analyze the kinetic properties of a
Michaelis enzyme by measuring its velocity of catalysis at a
range of substrate concentrations and plotting [S] vs V, or 1/[S]
vs 1/V. You can also analyze the properties of an enzyme
inhibitor by carrying out the same experiment and graphic
analysis in the presence of the inhibitor. If you do this for a
noncompetitive inhibitor, what difference will you see when you
compare the enzyme kinetics to an uninhibited control?
Vmax and KM both remain the same with a non-competitive
inhibitor.
Vmax will decrease and KM will increase.
Vmax and KM will both decrease.
Vmax will be unchanged but KM will increase.
Vmax will decrease but KM will be unchanged. Correct
Answers Vmax will decrease but KM will be unchanged.
Assume that a spherical cell, with a diameter of 20 μm, has
spherical mitochondria, each with a diameter of 0.2 μm. If
mitochondria occupy 10% of the volume of the cell, how many
mitochondria are there? Correct Answers 100,000
, Assuming you have 10mL of a solution at pH 5 that is not
buffered, how much water would you need to add to the solution
in order to raise the pH to 6? Render your answer in mL
(milliliters). Correct Answers 90
Cells can take up molecules from the outside world by
endocytosis, through membrane channels, or by simple
diffusion. But regardless of the avenue, the rate at which the cell
can acquire material from the outside world is limited by the
total surface area of the cell. On the other hand, the amount of
material that they need to take up is proportional to the cell
volume - i.e., how much biomass is in the cell. Some cells are
spherical. Correct Answers 0.3
Cells in the body of a metazoan organism like yourself have
many shapes and sizes that are suited to their particular
functions. Consider a muscle cell, which has the shape of a long
cylinder. Assume a cell in one of the tiny dorsal interosseous
muscles of your hand is 14 mm long, with a radius of 12 μm.
What is its surface-to-volume ratio in μm-1? Correct Answers
0.1668
Consider the reaction:
Glucose + Pi ⇔ Glucose-6-phosphate + H20 Assume ΔGo =
+12.8 kJ/mol
What is the equilibrium constant, Keq, for this reaction? Correct
Answers 0.0056
Consider this equation:
Glucose + Pi ⇔ Glucose-6-phosphate + H20 ΔGo = +12.5
kJ/mol
