PRINCIPLES OF BIOCHEMISTRY
COMPREHENSIVE TEST PAPER QUESTIONS
AND SOLUTIONS
●● DNA contains 2 antiparallel strands of nucleotides joined by
Answer: 3'->5' phosphodiester linkages
●● Eukaryotic DNA molecules are packaged with histones to form
___________, which is the fundamental unit of chromatin
Answer: a nucleosome
●● What is a nucleosome composed of?
Answer: histones H2A, H2B, H3, H4 (two molecules each) and 146 bp
of DNA
●● Name this type of RNA:
Carries genetic information from the gene to a ribosome, where the
corresponding protein can be synthesized
Answer: mRNA
●● Name this type of RNA:
,An integral part of ribosomes, accounts for ~80% of RNA in cells
Answer: rRNA
●● Name this type of RNA:
Carries activated amino acids to ribosomes for polypeptide synthesis
Answer: tRNA
●● Name this type of RNA:
has catalytic activity (catalyze cleavage of RNA) or associates with
proteins to enhance activity
Answer: small RNA
●● DNA double helix unwinds at a specific point known as the
Answer: origin of replication (Ori)
●● Prokaryotes have ______ (one/multiple) Ori, while Eukaryotes have
________ (one/multiple)
Answer: one; multiple
●● DNA replication is ________ (unidirectional/ bidirectional)
, Answer: bidirectional
●● What is the function of DNA polymerase III?
Answer: synthesizes new DNA strand
●● What is the function of DNA polymerase I?
Answer: fills gaps; excision of primers
●● What is the function of single stranded DNA binding proteins
(SSBs)?
Answer: stabilize separated DNA strands
●● What is the function of primase?
Answer: synthesize short RNA primers
●● This term refers to the process by which RNA polymerase
synthesizes RNA from a DNA template
Answer: transcription
●● The 5'-terminal cap structure of eukaryotic mRNA is a(n)
Answer: 7-methylguanosine joined to the mRNA via a 5' ->5'
triphosphate linkgage
COMPREHENSIVE TEST PAPER QUESTIONS
AND SOLUTIONS
●● DNA contains 2 antiparallel strands of nucleotides joined by
Answer: 3'->5' phosphodiester linkages
●● Eukaryotic DNA molecules are packaged with histones to form
___________, which is the fundamental unit of chromatin
Answer: a nucleosome
●● What is a nucleosome composed of?
Answer: histones H2A, H2B, H3, H4 (two molecules each) and 146 bp
of DNA
●● Name this type of RNA:
Carries genetic information from the gene to a ribosome, where the
corresponding protein can be synthesized
Answer: mRNA
●● Name this type of RNA:
,An integral part of ribosomes, accounts for ~80% of RNA in cells
Answer: rRNA
●● Name this type of RNA:
Carries activated amino acids to ribosomes for polypeptide synthesis
Answer: tRNA
●● Name this type of RNA:
has catalytic activity (catalyze cleavage of RNA) or associates with
proteins to enhance activity
Answer: small RNA
●● DNA double helix unwinds at a specific point known as the
Answer: origin of replication (Ori)
●● Prokaryotes have ______ (one/multiple) Ori, while Eukaryotes have
________ (one/multiple)
Answer: one; multiple
●● DNA replication is ________ (unidirectional/ bidirectional)
, Answer: bidirectional
●● What is the function of DNA polymerase III?
Answer: synthesizes new DNA strand
●● What is the function of DNA polymerase I?
Answer: fills gaps; excision of primers
●● What is the function of single stranded DNA binding proteins
(SSBs)?
Answer: stabilize separated DNA strands
●● What is the function of primase?
Answer: synthesize short RNA primers
●● This term refers to the process by which RNA polymerase
synthesizes RNA from a DNA template
Answer: transcription
●● The 5'-terminal cap structure of eukaryotic mRNA is a(n)
Answer: 7-methylguanosine joined to the mRNA via a 5' ->5'
triphosphate linkgage
