UIUC MCB 100 Exam 4 Questions and Correct
Answers
What is bio-safety level 1 and what are the precautions?
- handling pathogens that do not cause disease in healthy humans
- hand-washing and disinfecting surfaces, no eating
What is bio-safety level 2 and what are the precautions?
- handling of moderately biohazardous agents in biosafety cabinets
- use PPE, limited to trained personnel, careful with sharps
What are microbes considered biosafety level 2?
bordetella pertussis (whooping cough), e. coli, salmonella, staphylococcus
What is bio-safety level 3 and what are the precautions?
- handling of highly pathogenic, infectious, or toxic microbes in biosafety cabinets with high
PPE
- HEPA filtered rooms, double-set doors to prevent air escape
What are microbes considered biosafety level 3?
anthrax, COVID, flu, TB, plague
What is bio-safety level 4 and what are the precautions?
- handling of highly infectious pathogens that cause deadly disease with no treatments
- separate, isolated buildings, special PPE suits, airlocks, UV light rooms, pressurized
,_______ is the appropriate laboratory practices for conducting research with
moderately bio-hazardous microbes in biosafety cabinets while wearing proper PPE
(personal protective equipment).
A. BSL5
B. BSL4
C. BSL3
D. BSL2
E. BSL1
D
Sterilization
removal of all forms of life from object or fluid through chemicals, irradiation, high pressure,
or filtration. considered aseptic
How is sterilization different from disinfection, sanitization, and pasteurization?
Sterilization eradicates all microbes, the others just reduce the microbial load
What are natural agents for disinfection?
antibiotics, natural acids like vinegar, alcohols
What are chemical agents for disinfection?
chemicals, drugs, detergents, gases
What can be used externally on inanimate objects?
disinfectants and sanitizers
What can be used on body surfaces?
antiseptics and germicides
, What can be used internally/ingested?
antibiotics, antimicrobials
What are anti-microbials?
substances that kill or inhibit the growth of microbes such as bacteria, fungi, or parasites
microbicidal vs. microbiostatic
What are microbiocidals?
germicidals that lyse and kill bacteria (can potentially release toxins)
What are microbiostatics?
prevents the growth of microbes but does not kill it, bacteria can recover and regrow when
antibiotics stop
Which of the following is bacteriostatic?
A. heating in an oven at 171°C for an hour
B. filtering through a 0.2 μm membrane
C. freezing below 0°C for 10 minutes
D. bleaching for an hour
E. boiling for an hour
C
What is microbial death?
the permanent loss of reproductive ability under ideal environmental conditions
What is microbial death rate?
often a constant rate for any given microbe under a particular set of conditions
Answers
What is bio-safety level 1 and what are the precautions?
- handling pathogens that do not cause disease in healthy humans
- hand-washing and disinfecting surfaces, no eating
What is bio-safety level 2 and what are the precautions?
- handling of moderately biohazardous agents in biosafety cabinets
- use PPE, limited to trained personnel, careful with sharps
What are microbes considered biosafety level 2?
bordetella pertussis (whooping cough), e. coli, salmonella, staphylococcus
What is bio-safety level 3 and what are the precautions?
- handling of highly pathogenic, infectious, or toxic microbes in biosafety cabinets with high
PPE
- HEPA filtered rooms, double-set doors to prevent air escape
What are microbes considered biosafety level 3?
anthrax, COVID, flu, TB, plague
What is bio-safety level 4 and what are the precautions?
- handling of highly infectious pathogens that cause deadly disease with no treatments
- separate, isolated buildings, special PPE suits, airlocks, UV light rooms, pressurized
,_______ is the appropriate laboratory practices for conducting research with
moderately bio-hazardous microbes in biosafety cabinets while wearing proper PPE
(personal protective equipment).
A. BSL5
B. BSL4
C. BSL3
D. BSL2
E. BSL1
D
Sterilization
removal of all forms of life from object or fluid through chemicals, irradiation, high pressure,
or filtration. considered aseptic
How is sterilization different from disinfection, sanitization, and pasteurization?
Sterilization eradicates all microbes, the others just reduce the microbial load
What are natural agents for disinfection?
antibiotics, natural acids like vinegar, alcohols
What are chemical agents for disinfection?
chemicals, drugs, detergents, gases
What can be used externally on inanimate objects?
disinfectants and sanitizers
What can be used on body surfaces?
antiseptics and germicides
, What can be used internally/ingested?
antibiotics, antimicrobials
What are anti-microbials?
substances that kill or inhibit the growth of microbes such as bacteria, fungi, or parasites
microbicidal vs. microbiostatic
What are microbiocidals?
germicidals that lyse and kill bacteria (can potentially release toxins)
What are microbiostatics?
prevents the growth of microbes but does not kill it, bacteria can recover and regrow when
antibiotics stop
Which of the following is bacteriostatic?
A. heating in an oven at 171°C for an hour
B. filtering through a 0.2 μm membrane
C. freezing below 0°C for 10 minutes
D. bleaching for an hour
E. boiling for an hour
C
What is microbial death?
the permanent loss of reproductive ability under ideal environmental conditions
What is microbial death rate?
often a constant rate for any given microbe under a particular set of conditions
