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MCB 101 MIDTERM EXAM QUESTIONS WITH VERIFIED ANSWERS

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MCB 101 MIDTERM EXAM QUESTIONS WITH VERIFIED ANSWERS

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MCB 101 MIDTERM EXAM QUESTIONS WITH VERIFIED
ANSWERS


Correct 57

Incorrect 00


Your answers


1 of 57

Definition



Lactic acid, ethanol, and sometimes acetic acid



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What important thing that you should
What is the source of bacteria for the
always do when you come into lab
cabbage fermentation exercise?
should you not do before this lab?




What products produced by
heterolactic fermentation inhibit
What is the main difference between
the growth of undesirable
homolactic and heterolactic
microorganisms in the
fermentation?
fermentation of cabbage to
sauerkraut?


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, 2 of 57

Definition



Gently pressing it against a flat, dry surface



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What should you do when you first
positive/simple stain characteristics
pick up your inoculating loop?




How will you inoculate your What is the source of bacteria for the
RODAC plate? cabbage fermentation exercise?


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3 of 57

Definition



Reduction of microbial diversity allows an oppurtunistic pathogen to
overgrow



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Per the general formula (not simplified) for glycolysis in the manual, there is a
required input of _________and generates a output of______________

, How are DNases secreted by pathogenic bacteria hypothesized to counteract
aspects of the immune system response?



A 68 year old woman is given a two week course of antibiotics to treat an
infected tooth. Shortly after completing the first week of antibiotics, she
develops persistent diarrhea that does not resolve for 3 weeks. How might use
of antibiotics have contributed to the development of diarrhea in this woman?




Egg yolk agar is able to test for the production of proteinase, lipase, and lecithinase.
After incubation, the egg yolk plate has a zone of inhibition around bacterial growth
and there is an obvious opalescent sheen on the surface of the bacterial growth.
What enzyme(s) does this bacterium produce?


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4 of 57

Definition



The generation of a non-hospitable environment by useful bacteria
that will not support growth of many pathogenic organisms



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Select the correct decolorizer for
each type of staining.
What is the main mechanism by
which humans utilize bacterial - negative stain
fermentation to preserve food? - spore stain
- acid fast stain
- gram stain

, Why is it necessary to stain bacterial What actions are taken during the
cells in order to view them using cabbage fermentation exercise to
brightfield microscopy? limit the growth of fungi?


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5 of 57

Definition



within an organism's environment



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What properties of MRS media make
Which exoenzyme would most likely
it ideal for the growth of
be secreted by a pathogen?
Lactobacillus.




Why is it important to allow a
The exoenzymes a bacterium
sterilized inoculating loop to cool
produces are often dictated by
before attempting to sample liquid
the resources available
culture?


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6 of 57

Definition

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