MCB 101 FINAL EXAM QUESTIONS WITH VERIFIED
ANSWERS
Lactose - Answers - Differential
Yeast Extract - Answers - Neither
Lysine - Answers - Differential
Agar - Answers - Not selective or differential
Which media will you use to start your isolation of Salmonella? - Answers - XLD Agar
How many isolated colonies will you streak onto your Salmonella Chromagenic agar
plate? - Answers - 4
How should Salmonella colonies appear on MacConkey media? - Answers - Beige
How should Salmonella colonies appear on XLD media? - Answers - Red with black
center
How should Salmonella colonies appear on Salmonella Chromagenic media? - Answers
- Purple
What gene will you attempt to amplify from your isolated bacteria to determine if they
are salmonella? - Answers - Invasin A
If you have been struggling to isolate your Gram positive bacterium, what media should
you ask your TA to let you use? - Answers - Phenylethanol Agar
How many colony types should you see on your first streak plates after they have been
incubated? - Answers - 2
How long will you incubate your biochemical tests before scoring them? - Answers - 48
hours
Below is a portion of your hypothetical dichotomous key for one of your unknown
bacteria. Your TA has given you approval to start your biochemical tests. How many
Kliger slants will you start based on this dichotomous key? - Answers - 1
How will you start your biochemical tests once your TA has approved your dichotomous
key? - Answers - One of each test listed in your dichotomous key started on the same
day
, After you set up your antibiotic dilution series for determining the MIC of tetracycline,
what concentration of tetracycline should be present in tube #9 (in μg/mL) if you
followed the directions correctly? - Answers - 0.3900
Which tube in the antibiotic dilution series for determing the MIC of tetracycline contains
no antibiotic? - Answers - 15
How much culture will you use for your spread plates to determine the MBC of
tetracycline? - Answers - 1000 μL
How many Luria plates will you use to determine the MBC of tetracycline in E. coli? -
Answers - One plate for each MIC tube that did not have growth
How many different bacteria species will you inoculate on to each antibiotic containg
plate as part of the Antibiotic Susceptibility exercise? - Answers - 4
Why must you centrifuge the donor strain, discard the media it was originally grown in,
and resuspend it in fresh Luria broth prior to mixing it with the recipient strain? -
Answers - The donor strain is grown in media containing the antibiotic kanamycin which
would inhibit the growth of the recipient if not removed.
How will you harvest the mating mix (mixture of donor and recipient) after it has been
incubated? - Answers - Pouring sterile Luria broth on the plate and creating a slurry
using your plate spreader.
What volume of mating mix (mixture of donor and recipient) will you spread plate on to a
Luria Agar + DAP plate? - Answers - 200 μL
On which type of media will only successfully transformed recipient cells grow? -
Answers - Luria Agar + Kanamycin
How many control plates will you start on Day 1 to evaluate the characteristics of the
donor and recipient strains? - Answers - 4
Amino Acid or Pigment Auxotroph--Serratia marcescens MS10 - Answers - Pigment
Amino Acid or Pigment Auxotroph--Serratia marcescens RWDE2 - Answers - Pigment
Amino Acid or Pigment Auxotroph--Serratia marcescens WB34 - Answers - Amino Acid
Amino Acid or Pigment Auxotroph--Serratia marcescens WB32 - Answers - Amino Acid
In the prodigiosin cross-feeding experiment of this lab, you will receive a LA Agar plate
and three strains of pigment mutant S. marcescens. Select the correct description of the
plating procedure. - Answers - Inoculate each strain as a single line, forming a tringle
ANSWERS
Lactose - Answers - Differential
Yeast Extract - Answers - Neither
Lysine - Answers - Differential
Agar - Answers - Not selective or differential
Which media will you use to start your isolation of Salmonella? - Answers - XLD Agar
How many isolated colonies will you streak onto your Salmonella Chromagenic agar
plate? - Answers - 4
How should Salmonella colonies appear on MacConkey media? - Answers - Beige
How should Salmonella colonies appear on XLD media? - Answers - Red with black
center
How should Salmonella colonies appear on Salmonella Chromagenic media? - Answers
- Purple
What gene will you attempt to amplify from your isolated bacteria to determine if they
are salmonella? - Answers - Invasin A
If you have been struggling to isolate your Gram positive bacterium, what media should
you ask your TA to let you use? - Answers - Phenylethanol Agar
How many colony types should you see on your first streak plates after they have been
incubated? - Answers - 2
How long will you incubate your biochemical tests before scoring them? - Answers - 48
hours
Below is a portion of your hypothetical dichotomous key for one of your unknown
bacteria. Your TA has given you approval to start your biochemical tests. How many
Kliger slants will you start based on this dichotomous key? - Answers - 1
How will you start your biochemical tests once your TA has approved your dichotomous
key? - Answers - One of each test listed in your dichotomous key started on the same
day
, After you set up your antibiotic dilution series for determining the MIC of tetracycline,
what concentration of tetracycline should be present in tube #9 (in μg/mL) if you
followed the directions correctly? - Answers - 0.3900
Which tube in the antibiotic dilution series for determing the MIC of tetracycline contains
no antibiotic? - Answers - 15
How much culture will you use for your spread plates to determine the MBC of
tetracycline? - Answers - 1000 μL
How many Luria plates will you use to determine the MBC of tetracycline in E. coli? -
Answers - One plate for each MIC tube that did not have growth
How many different bacteria species will you inoculate on to each antibiotic containg
plate as part of the Antibiotic Susceptibility exercise? - Answers - 4
Why must you centrifuge the donor strain, discard the media it was originally grown in,
and resuspend it in fresh Luria broth prior to mixing it with the recipient strain? -
Answers - The donor strain is grown in media containing the antibiotic kanamycin which
would inhibit the growth of the recipient if not removed.
How will you harvest the mating mix (mixture of donor and recipient) after it has been
incubated? - Answers - Pouring sterile Luria broth on the plate and creating a slurry
using your plate spreader.
What volume of mating mix (mixture of donor and recipient) will you spread plate on to a
Luria Agar + DAP plate? - Answers - 200 μL
On which type of media will only successfully transformed recipient cells grow? -
Answers - Luria Agar + Kanamycin
How many control plates will you start on Day 1 to evaluate the characteristics of the
donor and recipient strains? - Answers - 4
Amino Acid or Pigment Auxotroph--Serratia marcescens MS10 - Answers - Pigment
Amino Acid or Pigment Auxotroph--Serratia marcescens RWDE2 - Answers - Pigment
Amino Acid or Pigment Auxotroph--Serratia marcescens WB34 - Answers - Amino Acid
Amino Acid or Pigment Auxotroph--Serratia marcescens WB32 - Answers - Amino Acid
In the prodigiosin cross-feeding experiment of this lab, you will receive a LA Agar plate
and three strains of pigment mutant S. marcescens. Select the correct description of the
plating procedure. - Answers - Inoculate each strain as a single line, forming a tringle
