MLT ASCP BOARD EXAM HIGH-YIELD
REVISION PRACTICE TEST
QUESTIONS WITH CORRECT
ANSWERS GRADED A+ FOR 2026
ASSURED EXCEL!!
MLS/MLT ASCP BOARD EXAM HIGH-YIELD REVISION NOTES (Part 3)
Hematology, Chemistry, Immunology, Microbiology & Blood Bank Review
1. Glucose Dilution Calculation
Question: A glucose sample is diluted using 0.25 mL patient sample + 750 µL diluent. Analyzer
result = 325 mg/dL.
CORRECT ANSWER: B. 1300 mg/dL
Calculation:
• Total volume = 1000 µL
• Patient sample = 250 µL
• Dilution = 1:4
• Final result = 325 × 4
1300 mg/dL
2. Christensen's Urea Agar
Question: Pink slant only reaction indicates?
CORRECT ANSWER: A. Weak activity
Key Points
• Slant only pink = weak urease producer
, • Slant + butt pink = strong urease producer
3. First Step of PCR
CORRECT ANSWER: D. Denaturation
PCR Steps
1. Denaturation
2. Annealing (Hybridization)
3. Extension
4. Isotonic Saline Concentration
CORRECT ANSWER: B. 0.85% NaCl
5. Disseminated Intravascular Coagulation (DIC)
CORRECT ANSWER: C. Prolonged PT, decreased fibrinogen, decreased platelets, increased
FDP
DIC Findings
⬆ PT
⬆ aPTT
⬆ FDP/D-Dimer
⬇ Platelets
⬇ Fibrinogen
6. Routine Sperm Count Dilution
CORRECT ANSWER: B. 1:20
7. Prozone Effect
CORRECT ANSWER: B. False Negative
REVISION PRACTICE TEST
QUESTIONS WITH CORRECT
ANSWERS GRADED A+ FOR 2026
ASSURED EXCEL!!
MLS/MLT ASCP BOARD EXAM HIGH-YIELD REVISION NOTES (Part 3)
Hematology, Chemistry, Immunology, Microbiology & Blood Bank Review
1. Glucose Dilution Calculation
Question: A glucose sample is diluted using 0.25 mL patient sample + 750 µL diluent. Analyzer
result = 325 mg/dL.
CORRECT ANSWER: B. 1300 mg/dL
Calculation:
• Total volume = 1000 µL
• Patient sample = 250 µL
• Dilution = 1:4
• Final result = 325 × 4
1300 mg/dL
2. Christensen's Urea Agar
Question: Pink slant only reaction indicates?
CORRECT ANSWER: A. Weak activity
Key Points
• Slant only pink = weak urease producer
, • Slant + butt pink = strong urease producer
3. First Step of PCR
CORRECT ANSWER: D. Denaturation
PCR Steps
1. Denaturation
2. Annealing (Hybridization)
3. Extension
4. Isotonic Saline Concentration
CORRECT ANSWER: B. 0.85% NaCl
5. Disseminated Intravascular Coagulation (DIC)
CORRECT ANSWER: C. Prolonged PT, decreased fibrinogen, decreased platelets, increased
FDP
DIC Findings
⬆ PT
⬆ aPTT
⬆ FDP/D-Dimer
⬇ Platelets
⬇ Fibrinogen
6. Routine Sperm Count Dilution
CORRECT ANSWER: B. 1:20
7. Prozone Effect
CORRECT ANSWER: B. False Negative