CLINICAL PRACTICUM EXAM 2026 QUESTIONS
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CLINICAL PRACTICUM EXAM 2026 QUESTIONS
It takes approx. ____ from the time a tissue sample is received until it is
delivered to the pathologist.
48 hours
process of histotechnology?
Fixation, Accessioning, Gross Dissection and description, processing, embedding,
cutting and sliding, staining, delivery to pathologist
What is the order of events in histotechnology?
Accessioning, Gros Exam, Fixation, Processing (Sub steps: Dehydration, Clearing,
Impregnation), Embedding, Sectioning, Staining
What is the maximum thickness of tissue slices for decalcification?
4-5mm
What is the typical washing duration after decalcification?
20-30 minutes on running tap water.
What are common acids used in acid decalcification?
Formic acid, nitric acid, hydrochloric acid, formaldehyde/formic acid, proprietary
decalcifers and other weak organic acids.
What is the process of decalcification
selection of tissue, fixation, decalcification, neutralization, washing, processing
What are the 2 different mechanisms of nuclear staining
Basic (cationic or +charged) dyes, Dyes with metal mordants
Nuclear staining is done with
basic dyes or dyes with a mordant
Cytoplasmic Staining is caused by
proteins or charged groups of amino acids
What are some bluing agents
scotts tape water, lithium carbonate, ammonia water, plain tap water
Differentiating agent
1% HCL in 70% alcohol
What is the sequence for H&E