CHEM 210
OBJECTIVE ASSESSMENT - EXAM
BioChem 210
Module 7 Exam
2026/2027
50 100% 2026/2027
Portage Learning | Bioenergetics
QUESTIONS andANSWERS
VERIFIED Metabolic Pathways: Part I
EDITION
TOPICS COVERED
Metabolic Flux and Regulation Fatty Acid Beta-Oxidation
Glycolysis Pathway Electron Transport Chain
Citric Acid Cycle Oxidative Phosphorylation
COVER PAGE - 1
,SECTION 1 | Metabolic Terminology and Bioenergetics | Q1-Q5 | BioChem 210 2026/2027
Q1 Question 1 of 50
A 34-year-old marathon runner is studying how her muscles regulate ATP
production during a race. She learns that metabolic flux refers to the rate of
metabolite flow through a pathway. If glycolytic flux suddenly increases in her
skeletal muscle, which outcome is most likely to occur?
A. The concentration of glucose-6-phosphate will decrease rapidly.
B. The rate of pyruvate formation will increase to match the higher flux.
C. The pH of the muscle cytosol will become strongly alkaline.
D. Allosteric inhibition of phosphofructokinase will permanently stop the pathway.
Correct Answer: B
Rationale:
Metabolic flux is the rate of flow through a pathway; increasing flux means more substrate is converted
to product per unit time, so pyruvate formation increases. Glucose-6-phosphate would initially rise, not
fall, and PFK-1 is activated, not inhibited, by AMP during exercise.
Q2 Question 2 of 50
A biochemistry student is analyzing a reaction with a delta G of +52 kJ/mol under
standard conditions. The student concludes the reaction is spontaneous. Which
statement correctly evaluates this conclusion?
A. The conclusion is correct because any reaction with a defined delta G will proceed forward.
B. The conclusion is incorrect because a positive delta G indicates the reaction is
endergonic and non-spontaneous.
C. The conclusion is incorrect because delta G values are only valid for reactions inside
mitochondria.
D. The conclusion is correct if the reaction is coupled to ATP hydrolysis, regardless of the delta
G sign.
Correct Answer: B
Rationale:
A positive delta G indicates an endergonic reaction that is non-spontaneous under standard conditions
and requires energy input. The sign of delta G directly determines spontaneity; positive values mean the
forward reaction is not favored.
BioChem 210 - 2026/2027 | Passing Score: 80% | Page 2 of 22
, Q3 Question 3 of 50
During a laboratory experiment, a researcher measures the delta G of a cellular
reaction and finds it is -78 kJ/mol. Which interpretation of this value is most
accurate for the reaction inside the cell?
A. The reaction is non-spontaneous and will require ATP hydrolysis to proceed.
B. The reaction is at equilibrium and will show no net change in metabolite concentrations.
C. The reaction is exergonic and spontaneous, releasing free energy that can drive
cellular work.
D. The reaction is endergonic because the negative value indicates energy absorption.
Correct Answer: C
Rationale:
A negative delta G indicates an exergonic, spontaneous reaction that releases free energy. This energy
can be harnessed to perform cellular work such as ATP synthesis or transport processes.
Q4 Question 4 of 50
A graduate student is comparing catabolic and anabolic pathways in liver cells. She
notes that catabolic pathways degrade complex molecules into simpler ones. Which
characteristic best distinguishes a catabolic pathway from an anabolic pathway?
A. Catabolic pathways consume ATP to build macromolecules from monomers.
B. Catabolic pathways are oxidative and typically release energy, often generating ATP
or NADH.
C. Catabolic pathways only occur in the cytosol and never involve oxygen.
D. Catabolic pathways require NADPH as the primary electron carrier for reduction reactions.
Correct Answer: B
Rationale:
Catabolic pathways break down molecules and release energy, producing reduced cofactors like NADH
and FADH2 that feed into the electron transport chain. Anabolic pathways build molecules and consume
energy, typically using ATP.
BioChem 210 - 2026/2027 | Passing Score: 80% | Page 3 of 22
OBJECTIVE ASSESSMENT - EXAM
BioChem 210
Module 7 Exam
2026/2027
50 100% 2026/2027
Portage Learning | Bioenergetics
QUESTIONS andANSWERS
VERIFIED Metabolic Pathways: Part I
EDITION
TOPICS COVERED
Metabolic Flux and Regulation Fatty Acid Beta-Oxidation
Glycolysis Pathway Electron Transport Chain
Citric Acid Cycle Oxidative Phosphorylation
COVER PAGE - 1
,SECTION 1 | Metabolic Terminology and Bioenergetics | Q1-Q5 | BioChem 210 2026/2027
Q1 Question 1 of 50
A 34-year-old marathon runner is studying how her muscles regulate ATP
production during a race. She learns that metabolic flux refers to the rate of
metabolite flow through a pathway. If glycolytic flux suddenly increases in her
skeletal muscle, which outcome is most likely to occur?
A. The concentration of glucose-6-phosphate will decrease rapidly.
B. The rate of pyruvate formation will increase to match the higher flux.
C. The pH of the muscle cytosol will become strongly alkaline.
D. Allosteric inhibition of phosphofructokinase will permanently stop the pathway.
Correct Answer: B
Rationale:
Metabolic flux is the rate of flow through a pathway; increasing flux means more substrate is converted
to product per unit time, so pyruvate formation increases. Glucose-6-phosphate would initially rise, not
fall, and PFK-1 is activated, not inhibited, by AMP during exercise.
Q2 Question 2 of 50
A biochemistry student is analyzing a reaction with a delta G of +52 kJ/mol under
standard conditions. The student concludes the reaction is spontaneous. Which
statement correctly evaluates this conclusion?
A. The conclusion is correct because any reaction with a defined delta G will proceed forward.
B. The conclusion is incorrect because a positive delta G indicates the reaction is
endergonic and non-spontaneous.
C. The conclusion is incorrect because delta G values are only valid for reactions inside
mitochondria.
D. The conclusion is correct if the reaction is coupled to ATP hydrolysis, regardless of the delta
G sign.
Correct Answer: B
Rationale:
A positive delta G indicates an endergonic reaction that is non-spontaneous under standard conditions
and requires energy input. The sign of delta G directly determines spontaneity; positive values mean the
forward reaction is not favored.
BioChem 210 - 2026/2027 | Passing Score: 80% | Page 2 of 22
, Q3 Question 3 of 50
During a laboratory experiment, a researcher measures the delta G of a cellular
reaction and finds it is -78 kJ/mol. Which interpretation of this value is most
accurate for the reaction inside the cell?
A. The reaction is non-spontaneous and will require ATP hydrolysis to proceed.
B. The reaction is at equilibrium and will show no net change in metabolite concentrations.
C. The reaction is exergonic and spontaneous, releasing free energy that can drive
cellular work.
D. The reaction is endergonic because the negative value indicates energy absorption.
Correct Answer: C
Rationale:
A negative delta G indicates an exergonic, spontaneous reaction that releases free energy. This energy
can be harnessed to perform cellular work such as ATP synthesis or transport processes.
Q4 Question 4 of 50
A graduate student is comparing catabolic and anabolic pathways in liver cells. She
notes that catabolic pathways degrade complex molecules into simpler ones. Which
characteristic best distinguishes a catabolic pathway from an anabolic pathway?
A. Catabolic pathways consume ATP to build macromolecules from monomers.
B. Catabolic pathways are oxidative and typically release energy, often generating ATP
or NADH.
C. Catabolic pathways only occur in the cytosol and never involve oxygen.
D. Catabolic pathways require NADPH as the primary electron carrier for reduction reactions.
Correct Answer: B
Rationale:
Catabolic pathways break down molecules and release energy, producing reduced cofactors like NADH
and FADH2 that feed into the electron transport chain. Anabolic pathways build molecules and consume
energy, typically using ATP.
BioChem 210 - 2026/2027 | Passing Score: 80% | Page 3 of 22
