Fully Solved.
MacConkey's Agar Plate (MAC) - Answer
Microbial Growth - Answer an increase in cell constituents that may or may not be
accompanied by an increase in cell number.
Balanced growth - Answer When all cell components are synthesized at constant rates
relative to one another.
Unbalanced Growth - Answer When the rates of synthesis of some components change
relative to the rates of synthesis of other components
General Growth Requirements - Answer Nutrients, Water, Temp, pH, appropriate
atmosphere, hydrostatic pressure, light
Barotolerant microbes - Answer Organisms that are not drastically affected by increased
pressure
Barophilic Microbes - Answer Organisms that require increased pressure for optimum growth
Nutrients - Answer materials that are required from the environment and are used for
growth and metabolism
Macronutrients - Answer - Required in large amounts
- C, N, H, O, S, P, and K, Ca, Mg and Fe
- Help maintain cell structure and metabolism
Elemental makeup of Proteins - Answer C, H, O, N, S
Elemental makeup of Lipids - Answer C, H, O, P
Elemental makeup of Carbohydrates - Answer C, H, O
Elemental makeup of Nucleic Acids - Answer C, H, O, N, P
, Micronutrients - Answer - Required in small amounts
- Mn, Zn, Cu
- Serve as cofactors for many enzymes
- Help enzyme function and maintain protein structure
Where to find micronutrients - Answer - In nature
- Lab glassware contamination
- Tap water
Ways organisms can acquire Carbon - Answer - Autotrophs: from CO2
- Heterotrophs: from pre-formed organic compounds.
Autotroph - Answer Organisms that make their own food
Heterotroph - Answer Organism that obtains energy from the food it consumes; ex:
carnivores
Photoautotrophs - Answer Organisms that use light as a source of energy and CO2 as carbon
source
Ways organisms can acquire Nitrogen - Answer - Organic compounds
- Nitrates
- Ammonium ions
- Sometimes N2 (nitrogen fixation)
Pure water availability (Aw) - Answer 1
Saturated salt water availability (Aw) - Answer 0.75
Some bugs and S. Aureus can tolerate _______ on our skin - Answer Salt levels
Organisms that can grow in habitats with low water activity by maintaining a high internal salt
concentration are called __________ organisms. - Answer Osmotolerant
Dried fruit water availability (Aw) - Answer 0.5