BACE STUDY GUIDE 2026 TEST PAPER
QUESTIONS AND SOLUTIONS GRADED A+
●● Assay: Types.
Answer: Immunoassays, drug assays, diagnostic assays, enzyme assays
●● ELISA.
Answer: A highly sensitive and specific method that relies on antibodies
binding to specific antigens
●● Western Blot.
Answer: Separates proteins by size and then uses antibodies to detect
specific proteins of interest
●● Chromatography.
Answer: A process of separating components of a mixture
-Dissolves a mixture of interest in a mobile phase and transports it
through a stationary phase
-Components of the mixture can be separated from one another based on
their different speeds of travel
●● Column Chromatography.
, Answer: A stationary phase in the form of a solid material packed in a
column and a mobile phase that flows through it to separate components
●● Ion Exchange Chromatography.
Answer: Separates molecules based on their charge
●● Affinity Chromatography.
Answer: Separates molecules based on specific binding interactions, like
antibodies and antigens
●● Size-Exclusion Chromatography.
Answer: Separates molecules based on their molecular weight as they
pass through a porous stationary phase
●● Hydrophobic Interaction Chromatography.
Answer: Separates ad purify proteins or peptides based on their
hydrophobic properties
●● Chromatography: Retention Time.
Answer: The amount of time a compound spends on the column after it
has been injected
●● Spectrophotometry.
QUESTIONS AND SOLUTIONS GRADED A+
●● Assay: Types.
Answer: Immunoassays, drug assays, diagnostic assays, enzyme assays
●● ELISA.
Answer: A highly sensitive and specific method that relies on antibodies
binding to specific antigens
●● Western Blot.
Answer: Separates proteins by size and then uses antibodies to detect
specific proteins of interest
●● Chromatography.
Answer: A process of separating components of a mixture
-Dissolves a mixture of interest in a mobile phase and transports it
through a stationary phase
-Components of the mixture can be separated from one another based on
their different speeds of travel
●● Column Chromatography.
, Answer: A stationary phase in the form of a solid material packed in a
column and a mobile phase that flows through it to separate components
●● Ion Exchange Chromatography.
Answer: Separates molecules based on their charge
●● Affinity Chromatography.
Answer: Separates molecules based on specific binding interactions, like
antibodies and antigens
●● Size-Exclusion Chromatography.
Answer: Separates molecules based on their molecular weight as they
pass through a porous stationary phase
●● Hydrophobic Interaction Chromatography.
Answer: Separates ad purify proteins or peptides based on their
hydrophobic properties
●● Chromatography: Retention Time.
Answer: The amount of time a compound spends on the column after it
has been injected
●● Spectrophotometry.