TEST BANK MOLECULAR DIAGNOSTICS 3RD
EDITION BUCKINGHAN (CHAPETR 1-15)
QUESTIONS AND CORRECT ANSWERS (VERIFIED
ANSWERS) Q&A 2026 |INSTANT DOWNLOAD PDF
1. What is the primary role of DNA in cells?
A. Energy storage
B. Protein synthesis template
C. Lipid formation
D. Cell membrane structure
Rationale: DNA stores genetic information used to make RNA
and proteins.
Correct Answer: B
2. Which bond holds the two DNA strands together?
A. Covalent bond
B. Ionic bond
C. Hydrogen bond
D. Peptide bond
Rationale: Hydrogen bonds connect complementary bases.
Correct Answer: C
3. RNA differs from DNA because it contains:
,A. Thymine
B. Deoxyribose
C. Uracil
D. Double strand
Rationale: RNA uses uracil instead of thymine.
Correct Answer: C
4. PCR is used to:
A. Destroy DNA
B. Amplify DNA
C. Translate proteins
D. Sequence proteins
Rationale: PCR exponentially amplifies DNA fragments.
Correct Answer: B
5. The enzyme used in PCR is:
A. DNA ligase
B. RNA polymerase
C. Taq polymerase
D. Restriction enzyme
Rationale: Taq polymerase withstands high temperatures.
Correct Answer: C
6. Denaturation in PCR occurs at:
,A. Low temperature
B. Medium temperature
C. High temperature
D. Room temperature
Rationale: Heat separates DNA strands.
Correct Answer: C
7. Annealing in PCR allows:
A. DNA breakdown
B. Primer binding
C. Protein synthesis
D. RNA degradation
Rationale: Primers bind to target sequences.
Correct Answer: B
8. Extension step in PCR involves:
A. DNA melting
B. Primer removal
C. DNA synthesis
D. RNA formation
Rationale: Polymerase extends new DNA strand.
Correct Answer: C
9. Primers are:
, A. Long DNA strands
B. Short DNA sequences
C. Proteins
D. Lipids
Rationale: Primers initiate DNA synthesis.
Correct Answer: B
10. Gel electrophoresis separates DNA based on:
A. Color
B. Size
C. Shape
D. Temperature
Rationale: Smaller fragments move faster.
Correct Answer: B
11. DNA moves toward the:
A. Positive electrode
B. Negative electrode
C. Neutral zone
D. Light source
Rationale: DNA is negatively charged.
Correct Answer: A
12. Agarose gel is used because it:
EDITION BUCKINGHAN (CHAPETR 1-15)
QUESTIONS AND CORRECT ANSWERS (VERIFIED
ANSWERS) Q&A 2026 |INSTANT DOWNLOAD PDF
1. What is the primary role of DNA in cells?
A. Energy storage
B. Protein synthesis template
C. Lipid formation
D. Cell membrane structure
Rationale: DNA stores genetic information used to make RNA
and proteins.
Correct Answer: B
2. Which bond holds the two DNA strands together?
A. Covalent bond
B. Ionic bond
C. Hydrogen bond
D. Peptide bond
Rationale: Hydrogen bonds connect complementary bases.
Correct Answer: C
3. RNA differs from DNA because it contains:
,A. Thymine
B. Deoxyribose
C. Uracil
D. Double strand
Rationale: RNA uses uracil instead of thymine.
Correct Answer: C
4. PCR is used to:
A. Destroy DNA
B. Amplify DNA
C. Translate proteins
D. Sequence proteins
Rationale: PCR exponentially amplifies DNA fragments.
Correct Answer: B
5. The enzyme used in PCR is:
A. DNA ligase
B. RNA polymerase
C. Taq polymerase
D. Restriction enzyme
Rationale: Taq polymerase withstands high temperatures.
Correct Answer: C
6. Denaturation in PCR occurs at:
,A. Low temperature
B. Medium temperature
C. High temperature
D. Room temperature
Rationale: Heat separates DNA strands.
Correct Answer: C
7. Annealing in PCR allows:
A. DNA breakdown
B. Primer binding
C. Protein synthesis
D. RNA degradation
Rationale: Primers bind to target sequences.
Correct Answer: B
8. Extension step in PCR involves:
A. DNA melting
B. Primer removal
C. DNA synthesis
D. RNA formation
Rationale: Polymerase extends new DNA strand.
Correct Answer: C
9. Primers are:
, A. Long DNA strands
B. Short DNA sequences
C. Proteins
D. Lipids
Rationale: Primers initiate DNA synthesis.
Correct Answer: B
10. Gel electrophoresis separates DNA based on:
A. Color
B. Size
C. Shape
D. Temperature
Rationale: Smaller fragments move faster.
Correct Answer: B
11. DNA moves toward the:
A. Positive electrode
B. Negative electrode
C. Neutral zone
D. Light source
Rationale: DNA is negatively charged.
Correct Answer: A
12. Agarose gel is used because it:
