MICROBIOLOGY UTA 2460 EXAM 3 FINAL
PAPER 2026 SOLVED QUESTIONS AND FULL
SOLUTION UPDATED
◉ Haemophilus influenzae must be grown on chocolate agar, which
is blood agar treated with heat to release growth factors in the
medium. H. influenzae is described as ___.
Answer: fastidious
◉ Direct count of total cells can be performed using a ________ or a
________.
Answer: hemocytometer or a Petroff-Hausser counting chamber
◉ The ________ method allows direct count of total cells growing on
solid medium.
Answer: plate count
◉ A statistical estimate of the number of live cells in a liquid is
usually done by ________.
Answer: most probable number
,◉ For this indirect method of estimating the growth of a culture, you
measure ________ using a spectrophotometer.
Answer: turbidity
◉ Active growth of a culture may be estimated indirectly by
measuring the following products of cell metabolism: ________ or
________.
Answer: ATP or acid from fermentation
◉ A bacterium that thrives in a soda lake where the average pH is
10.5 can be classified as a(n) ________.
Answer: alkaliphile
◉ Lactobacillus acidophilus grows best at pH 4.5. It is considered
a(n) ________.
Answer: acidophile
◉ A bacterium that thrives in the Great Salt Lake but not in fresh
water is probably a ________.
Answer: halophile
◉ Blood agar contains many unspecified nutrients, supports the
growth of a large number of bacteria, and allows differentiation of
, bacteria according to hemolysis (breakdown of blood). The medium
is ________ and ________.
Answer: complex and differential
◉ What is the microbial death curve?
Answer: measure of the percent killed, usually logarithmic
◉ What is the thermal death point?
Answer: lowest temperature that kills all microbes in 10 minutes
◉ What is the thermal death time?
Answer: the length of time to kill all microbes at a certain
temperature
◉ What is high-temp short-time pasteurization?
Answer: milk is heated to 72 C for 15 seconds and then refridgerated
◉ What is ultra high temperature pasteurization?
Answer: milk is heated to 138 C for 2 seconds and then is shelf
stable
◉ What is pascalization?
PAPER 2026 SOLVED QUESTIONS AND FULL
SOLUTION UPDATED
◉ Haemophilus influenzae must be grown on chocolate agar, which
is blood agar treated with heat to release growth factors in the
medium. H. influenzae is described as ___.
Answer: fastidious
◉ Direct count of total cells can be performed using a ________ or a
________.
Answer: hemocytometer or a Petroff-Hausser counting chamber
◉ The ________ method allows direct count of total cells growing on
solid medium.
Answer: plate count
◉ A statistical estimate of the number of live cells in a liquid is
usually done by ________.
Answer: most probable number
,◉ For this indirect method of estimating the growth of a culture, you
measure ________ using a spectrophotometer.
Answer: turbidity
◉ Active growth of a culture may be estimated indirectly by
measuring the following products of cell metabolism: ________ or
________.
Answer: ATP or acid from fermentation
◉ A bacterium that thrives in a soda lake where the average pH is
10.5 can be classified as a(n) ________.
Answer: alkaliphile
◉ Lactobacillus acidophilus grows best at pH 4.5. It is considered
a(n) ________.
Answer: acidophile
◉ A bacterium that thrives in the Great Salt Lake but not in fresh
water is probably a ________.
Answer: halophile
◉ Blood agar contains many unspecified nutrients, supports the
growth of a large number of bacteria, and allows differentiation of
, bacteria according to hemolysis (breakdown of blood). The medium
is ________ and ________.
Answer: complex and differential
◉ What is the microbial death curve?
Answer: measure of the percent killed, usually logarithmic
◉ What is the thermal death point?
Answer: lowest temperature that kills all microbes in 10 minutes
◉ What is the thermal death time?
Answer: the length of time to kill all microbes at a certain
temperature
◉ What is high-temp short-time pasteurization?
Answer: milk is heated to 72 C for 15 seconds and then refridgerated
◉ What is ultra high temperature pasteurization?
Answer: milk is heated to 138 C for 2 seconds and then is shelf
stable
◉ What is pascalization?
