MICROBIOLOGY UTA 2460 EXAM 3 CERTIFICATION EVALUATION 2026 FULL QUESTIONS AND CORRECT ANSWERS

MICROBIOLOGY UTA 2460 EXAM 3
CERTIFICATION EVALUATION 2026 FULL
QUESTIONS AND CORRECT ANSWERS

◉ 4 Basic Steps.
Answer: 1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division


◉ Cytokinesis.
Answer: Division of cytoplasm


◉ Z Ring Assembly.
Answer: FtsZ assembles Z ring to form divisome


◉ Divisome.
Answer: Cell division apparatus


◉ Generation Time.
Answer: (Doubling Time)-time it takes to double the population

,◉ Lag Phase.
Answer: Cells adjust to culture medium; no change in population


◉ Log Phase.
Answer: Binary fission occurs; cell replication > cell death


◉ Stationary Phase.
Answer: Resources become depleted; cell respiration=cell death


◉ Death Phase.
Answer: Endospores can form; cell replication < cell death


◉ Close System Cultures.
Answer: Have finite resources


◉ Open System Cultures.
Answer: -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology


◉ Microscopic Cell Count.

, Answer: Cells are counted under a microscope using a laser etched
slide


◉ Fluorescence Staining.
Answer: -Cells are counted under a microscope or flow cytometer
-Red stain binds to damaged cells


◉ Viable Cell Counts.
Answer: -Samples are diluted and grown on solid media


◉ Countable Range.
Answer: 30-300 CFU/ml


◉ Optical Density.
Answer: -Measured with spectrophotometer
-Light is passed thru culture and measured on other side


◉ Steps of Biofilm Formation.
Answer: 1. Attachment of planktonic cells
2. Cells become sessile
3. Growth & Division on substrate
4. Production of extracellular polymeric substance