UAMS-Microbiology Final Latest Questions and
Answers (2026) | Updated Comprehensive
Review with Detailed Rationales | A+ Verified
BEA: Black
Salt: Growth
Optochin: Resistant -✓✓Enterococcus
BEA: Black
Salt: No Growth
Optochin: resistant -✓✓GDS
BEA: Colorless
Salt: No Growth
Optochin: Susceptible -✓✓Streptococcus pneumoniae
BEA: Coloreless
Salt: No Growth
Optochin: Resistant -✓✓Viridans Strep
Hemolysis: Beta
Gram Stain: GP Coccobacilli
Catalase: +
BEA: +
CAMP/Hippurate: +
Motility: + @ 25 C -✓✓Listeria
What organisms are motile at 25 C? -✓✓Yersinia entercolitica & Listeria
What GPR organisms form spores? -✓✓Bacillus species (aerobic)
Clostridium species (anaerobic)
What temperature should CIN agar be incubated at? -✓✓RT
Selective for Gram-Negative Bacilli -✓✓EMB & MAC
Selective media for Gram-positive organisms -✓✓CNA & PEA
Gram staining steps -✓✓1. crystal violet
2. iodine
3. alcohol
4. safranin
, ID Of Staphylococci -✓✓-Slide coagulase
-Tube coagulase
-MSA
-Latex agglutination
-Novobiocin
Reagent: Bile Solubility -✓✓10% deoxycholate (bile)
Enhance growth of specific MOs, while inhibiting the growth of others (GN & Selenite) -
✓✓Enrichment Broths
No antimicrobial substances & allows growth of most organisms (BAP, CHOC) -
✓✓Nonselective Media
Contains antimicrobial substances, allows growth of specific bacteria while inhibiting the
growth of others (PEA, CNA) -✓✓Selective Media
Basic growth media for bacteria (BAP/ CHOC) -✓✓Nutrient Media
Metabolic differences and biochemical reactions in the media distinguishes MO (ph
Color indicator) -✓✓Differential Media
Reagent: Catalase -✓✓Hydrogen Peroxide
Reagent: Coagulase -✓✓Plasma
What is CHROMagar used for? -✓✓MRSA
Sticky Staph
Cat: =
Oxidase: =
Bacitracin: R -✓✓Rothia
Reagent: PYR -✓✓N,N dimethylaminocinnamaldehyde
What causes satelliting Strep? -✓✓Deficiency in Vitamin B6 (pyridoxal)
Gamma Hemolysis Strep Tests -✓✓-BEA
-Salt
-PYR
-CAMP/ Hipp
Alpha Hemolysis Strep Tests -✓✓-BEA
Answers (2026) | Updated Comprehensive
Review with Detailed Rationales | A+ Verified
BEA: Black
Salt: Growth
Optochin: Resistant -✓✓Enterococcus
BEA: Black
Salt: No Growth
Optochin: resistant -✓✓GDS
BEA: Colorless
Salt: No Growth
Optochin: Susceptible -✓✓Streptococcus pneumoniae
BEA: Coloreless
Salt: No Growth
Optochin: Resistant -✓✓Viridans Strep
Hemolysis: Beta
Gram Stain: GP Coccobacilli
Catalase: +
BEA: +
CAMP/Hippurate: +
Motility: + @ 25 C -✓✓Listeria
What organisms are motile at 25 C? -✓✓Yersinia entercolitica & Listeria
What GPR organisms form spores? -✓✓Bacillus species (aerobic)
Clostridium species (anaerobic)
What temperature should CIN agar be incubated at? -✓✓RT
Selective for Gram-Negative Bacilli -✓✓EMB & MAC
Selective media for Gram-positive organisms -✓✓CNA & PEA
Gram staining steps -✓✓1. crystal violet
2. iodine
3. alcohol
4. safranin
, ID Of Staphylococci -✓✓-Slide coagulase
-Tube coagulase
-MSA
-Latex agglutination
-Novobiocin
Reagent: Bile Solubility -✓✓10% deoxycholate (bile)
Enhance growth of specific MOs, while inhibiting the growth of others (GN & Selenite) -
✓✓Enrichment Broths
No antimicrobial substances & allows growth of most organisms (BAP, CHOC) -
✓✓Nonselective Media
Contains antimicrobial substances, allows growth of specific bacteria while inhibiting the
growth of others (PEA, CNA) -✓✓Selective Media
Basic growth media for bacteria (BAP/ CHOC) -✓✓Nutrient Media
Metabolic differences and biochemical reactions in the media distinguishes MO (ph
Color indicator) -✓✓Differential Media
Reagent: Catalase -✓✓Hydrogen Peroxide
Reagent: Coagulase -✓✓Plasma
What is CHROMagar used for? -✓✓MRSA
Sticky Staph
Cat: =
Oxidase: =
Bacitracin: R -✓✓Rothia
Reagent: PYR -✓✓N,N dimethylaminocinnamaldehyde
What causes satelliting Strep? -✓✓Deficiency in Vitamin B6 (pyridoxal)
Gamma Hemolysis Strep Tests -✓✓-BEA
-Salt
-PYR
-CAMP/ Hipp
Alpha Hemolysis Strep Tests -✓✓-BEA
