Glycolysis: 5.3 The Regulation of Glycolysis Test with Verified Answers Graded A+

Glycolysis: 5.3 The Regulation of Glycolysis Test with Verified Answers Graded A+


1. Glycolytic pathway is regulated at various points ;Feedback inhibition
through : ;Allosteric control.

2. Glycolytic pathway is tightly controlled with respect to ;Hexokinase
three important enzymes: ;Phosphofructokinase
(PFK)
;Pyruvate kinase.




3. Which enzyme is the primary regulatory enzyme (most Phosphofructokinase
important in Glycolysis (Glycolysis of skeletal muscle)? (PFK)




4. Why Phosphofructokinase (PFK) primary regulatory Ilt is responsible for cat-
enzyme (most important in Glycolysis)? alyzing the first irreversible
Rnx in the " Committed
Step"

lWhen‘level of ATP inhibit
PFK, which in turn cause a
build up of G6P which in-
hibit Hexokinase
and the excess of G6P is
used by the cell to make
Glycogen

5. Phosphofructokinase (PFK) I*t is responsible for cat-
alyzing the first irreversible


,Glycolysis: 5.3 The Regulation of Glycolysis Test with Verified Answers Graded A+


Rnx in the " Committed
Step" where
F6P öF1,6BF

*P F K is allosterically inhib-
ited by ‘level of ATP
*l e‘ vel of ATP lead to gly-
colysis halts the glycolytic
pathway




6. How does Phosphofructokinase (PFK) work? A* T P binds to specific site of
PFK (located in a ditterent
area than the active site).
Binding of ATP to this al-
losteric site will “enzyme
ability to bind to F6P

*B ut if‘level AMP, the
inhibitory process is re-
versed

7. How does AMP generate? A5denosine monophos-
phate (AMP) not directly
generated along the gly-
colic pathway

D5uring period of "in-
tense exercise", an en-