Nonsense Mutations
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singluar base is changed and causes early termination in the sequence adn
now causes a "stop" codon to form
Translocation
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DNA is excahnged in reciprocal way from one chromosome to another
Use mature mRNA as template
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Production of proteins
Splice Sites
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-Important in genetic disease
-Direct the spilicing machinery to cleave and rejoin the DNA frgmnry
Non classical DNA polymerases
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, Required to continue translation after repair
1. Zeta, Eta, Rev1
2. Iota, Kappa
3. Nu
4. Theta
5. Lambda and Mu
6. Terminal deoxynucleotide transferase
Creatuon of rDNA using DNA ligase
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-Cobined the Vector with insert DNA
-Uses PCR or restriction enzymes to join these
-Multipl cloning site is where it has the sequence of all the restrictio sites at
the point the vector or insert can cleave
-Vecotr and insert have to be cut by te restriciton enzyme so that they can
joins
-Needs ligase to covelenlty link them
Missense Mutation
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Changed in 1 base and causes a change in the amino acid that is expressed
2 Types: Conservative and Nonconservative
Hybridization Assays
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, -Exploit the specifity of zationhybridia
-Two single poly nucleotide DNA or RNA or oligonucleotide will stable
dsHybrid only if plenty of base matiching is present
-Can be be selected for perfect matching or two allow for varying degree
of mismatching
Non-ionzing
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-Ultra violet
-Cause covalent bonding between adjacent pyrimidines
Exposure to endogneous and exogenous sources
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-Typically refer to some type of chemical and chemical environments
Go
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Where cells can stop cycling for a period of time, can reeneter back into
the cell cycle
Give this one a try later!
singluar base is changed and causes early termination in the sequence adn
now causes a "stop" codon to form
Translocation
,Give this one a try later!
DNA is excahnged in reciprocal way from one chromosome to another
Use mature mRNA as template
Give this one a try later!
Production of proteins
Splice Sites
Give this one a try later!
-Important in genetic disease
-Direct the spilicing machinery to cleave and rejoin the DNA frgmnry
Non classical DNA polymerases
Give this one a try later!
, Required to continue translation after repair
1. Zeta, Eta, Rev1
2. Iota, Kappa
3. Nu
4. Theta
5. Lambda and Mu
6. Terminal deoxynucleotide transferase
Creatuon of rDNA using DNA ligase
Give this one a try later!
-Cobined the Vector with insert DNA
-Uses PCR or restriction enzymes to join these
-Multipl cloning site is where it has the sequence of all the restrictio sites at
the point the vector or insert can cleave
-Vecotr and insert have to be cut by te restriciton enzyme so that they can
joins
-Needs ligase to covelenlty link them
Missense Mutation
Give this one a try later!
Changed in 1 base and causes a change in the amino acid that is expressed
2 Types: Conservative and Nonconservative
Hybridization Assays
Give this one a try later!
, -Exploit the specifity of zationhybridia
-Two single poly nucleotide DNA or RNA or oligonucleotide will stable
dsHybrid only if plenty of base matiching is present
-Can be be selected for perfect matching or two allow for varying degree
of mismatching
Non-ionzing
Give this one a try later!
-Ultra violet
-Cause covalent bonding between adjacent pyrimidines
Exposure to endogneous and exogenous sources
Give this one a try later!
-Typically refer to some type of chemical and chemical environments
Go
Give this one a try later!
Where cells can stop cycling for a period of time, can reeneter back into
the cell cycle
