Medical Laboratory Scientist Board Review With Comprehensive
Practice Tests, Clinical Case Studies, Detailed Rationales, Laboratory
Science Notes & Verified Answers
Which Immunoassay Methodology Is The Emission Of Light By Molecules In An Excited State
With A Limited Amount Of Heat As The Result Of A Chemical Reaction?
Question
A. Chemiluminescence
B. Immunochromatography
C. Photometry
D. Spectrophotometry
Correct Answer: A. Chemiluminescence
Chemiluminescence Is The Emission Of Light By Molecules In An Excited State With A Limited
Amount Of Heat (Luminescence) As The Result Of A Chemical Reaction. Chemiluminescent
Assays Are Ultrasensitive And Are Widely Used In Automated Immunoassays And DNA Probe
Assay Systems. Chemiluminescence Has Excellent Sensitivity And Dynamic Range.
Chemiluminescent Labels Can Be Attached To An Antigen Or An Antibody. Most
Chemiluminescent Reagents And Conjugates Are Stable And Relatively Nontoxic.
What Characteristics/Functions Do Calibrators Have?
Question
A. Contains A Known Amount Of Analyte Being Tested
B. Monitors The Quality Of Reagents
C. Monitors The Quality Of The Sample
,D. Prevents Equipment Failure
Correct Answer: A. Contains A Known Amount Of Analyte Being Tested
Calibrators Are Used To Establish A Correlation Between The Measurement Response And The
Concentration Or Amount Of The Substance That Is Being Measured By The Test Procedure.
Absorbance Can Be Calculated Easily From Percent Transmission Using Which Calculation?
A.2 - Log10 %T
B.2 + Log10 %T
C.Log10 %T - 2
D.Log10 %T + 2
Correct Answer: A. 2 - Log10 %T
Absorbance Can Be Calculated Easily From Percent Transmission Using 2 - Log10 %T. If All The
Light Passes Through A Solution Without Any Absorption, Absorbance Is Zero And The %T Is
100%. If All The Light Is Absorbed, %T Is Zero, And Absorption Is Infinite.
What Is A Substance Or Constituent In Which The Laboratory Conducts Testing?
A. A Solution
B. An Additive
C. An Analyte
D. An Anticoagulant
Correct Answer: C. An Analyte
,In The Testing Process, Analytes Or Constituents Are Measured.
What Does A Common Application Of The Polarized Light Microscope In The Clinical Laboratory
Do?
Determine Fanas With Fluorescence
B. Determine Neutrophilic From Lymphocytic Leukemia
C. Examine Histochemical Reactions
D. Study Crystals In Urine And In Cells
Correct Answer: D. Study Crystals In Urine And Cells
What Is The Chelation Of Calcium In The EDTA Tube?
A. Maintains A Constant Temperature For Components
B. Prevents Coagulation By Inhibiting Coagulation
C. Prevents The Separation Of Blood Components
D. Stops The Lysing Of The Red Blood Cells
Correct Answer: B. Prevents Coagulation By Inhibiting Coagulation
CLSI Recommends The Salts Of The Chelating (Calcium Binding) Agent EDTA As The
Anticoagulant Of Choice For Blood Cell Counting And Sizing.
How Must A Laboratory Operate To Handle TB Sputum And TM Materials?
, A. Biosafety Level Of 2+ Or 3
B. Biosafety Level 4
C. Location Where Air Exchange Is Continuous
D. Location Where Fume Hood Exhausts Are Monitored Daily
Correct Answer: A. Biosafety Level Of 2+ Or 3
BSL-3 Builds Upon The Requirements Of BSL-2. Organisms That Can Cause Serious Or Potentially
Lethal Disease Through Respiratory Transmission Are Typically Worked With In A BSL-3
Laboratory. Example: Mycobacterium Tuberculosis.
What Are Class A Fire Extinguishers Used For?
A. Electric Equipment
B. Flammable Liquids
C. Ordinary Combustibles
D. Powdered Metal (Combustible) Material
Correct Answer: Ordinary Combustibles.
Class A Fire Extinguishers Are Classified As Ordinary Combustible. Additionally, Class A Fire
Extinguishers Have A Numerical Rating Based On UL-Conducted Tests And Designed To
Determine The Extinguishing Potential For Each Size And Type Of Extinguisher
Which Part Of The Microscope Directs And Focuses The Beam Of Light From The Bulb Onto The
Material Under Examination?