ASCP MB Review EXAM (2026) UPDATE Verified Questions And
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
primary structure - (answer)sequence of amino acids
secondary structure - (answer)side chain interactions (ie, alpha helices and beta-pleated sheets)
tertiary structure - (answer)configuration of folded proteins; if denatured, loses function
quaternary structure - (answer)functional association of separate proteins
UAA, UAG, UGA - (answer)nonsense/termination codons
amino acid charging - (answer)catalyzed by aminoacyl tRNA synthetase; Mg++ dependent
AUG - (answer)start codon (methionine)
size exclusion columns - (answer)porous beads that exclude larger molecules and retain smaller
molecules
single strand conformation polymorphism (SSCP) - (answer)short ds PCR products denatured then
rapidly cooled; fold into conformers; resolve on gel; mutations cause different conformation &
migration pattern
denaturing gradient gel electrophoresis (DGGE) - (answer)dsDNA fragments separated on PAGE with
gradient conc. of urea & formamide
sequence specific primer pcr (SSP-PCR) - (answer)primer 3' end falls on nucleotide to be analyzed; 3' end
must match template to extend by Taq; presence/absence of product = presence/absence of mutation
2.9 billion bp - (answer)size of human genome
,ASCP MB Review EXAM (2026) UPDATE Verified Questions And
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
polymorphism - (answer)change in dna sequence present in at least 1 - 2% of population
alpha satellite - (answer)highly repetitive sequences at centromere, interfere with chromosome
compaction, causes constriction at centromere
metacentric - (answer)chromosomal arms equal in length
sub-metacentric - (answer)one chromosome arm longer than other
acrocentric - (answer)one chromosome arm extremely small
telocentric - (answer)only 1 chromosome arm
robertsonian translocation - (answer)movement of most of one entire chromosome to centromere of
another
94 - 96°C - (answer)denaturation temp.
50 - 70°C - (answer)annealing temp.
68 - 75°C - (answer)elongation temp.
20 - 30bp - (answer)length of pcr primers
primer dimers - (answer)result from binding of primers onto each other through short (2-3 base)
homologies at 3' ends & copying of each primer sequence
0.1 - 0.5mM - (answer)dNTP concentration for PCR
, ASCP MB Review EXAM (2026) UPDATE Verified Questions And
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
1 - 4mM - (answer)MgCl2 concentration for PCR
positive control - (answer)ensures enzyme is active, buffer is optimal, primers are priming right
sequences, thermocycler is cycling properly
negative control (contamination control, reagent blank) - (answer)ensures reaction mix isn't
contaminated with template DNA or amplicons
negative template control - (answer)lacks target sequence, ensures primers not annealing to nontarget
sequence
amplification control - (answer)2nd set of primers and unrelated target, demonstrates that reaction is
working even if test sample not amplified
nested pcr - (answer)2 sets of primers used to amplify single target in 2 separate pcr runs
semi-nested pcr - (answer)one of 2nd round primers is same as 1st round
taqman probes - (answer)probe with dye at 5' end and quencher at 3' end; exonuclease activity of taq
degrades probe, separating dye and quencher allowing fluorescence
molecular beacons - (answer)measures product at annealing step; stem and loop probe with dye at 5'
end and quencher at 3' end; probe binds to template during annealing and fluoresces
scorpion-type primers - (answer)target-specific primers tailed at 5' end with quencher, stem loop and
fluorophore; after polymerization, secondary structure of primer overcome by hyb of primer to target,
allowing fluorescence
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
primary structure - (answer)sequence of amino acids
secondary structure - (answer)side chain interactions (ie, alpha helices and beta-pleated sheets)
tertiary structure - (answer)configuration of folded proteins; if denatured, loses function
quaternary structure - (answer)functional association of separate proteins
UAA, UAG, UGA - (answer)nonsense/termination codons
amino acid charging - (answer)catalyzed by aminoacyl tRNA synthetase; Mg++ dependent
AUG - (answer)start codon (methionine)
size exclusion columns - (answer)porous beads that exclude larger molecules and retain smaller
molecules
single strand conformation polymorphism (SSCP) - (answer)short ds PCR products denatured then
rapidly cooled; fold into conformers; resolve on gel; mutations cause different conformation &
migration pattern
denaturing gradient gel electrophoresis (DGGE) - (answer)dsDNA fragments separated on PAGE with
gradient conc. of urea & formamide
sequence specific primer pcr (SSP-PCR) - (answer)primer 3' end falls on nucleotide to be analyzed; 3' end
must match template to extend by Taq; presence/absence of product = presence/absence of mutation
2.9 billion bp - (answer)size of human genome
,ASCP MB Review EXAM (2026) UPDATE Verified Questions And
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
polymorphism - (answer)change in dna sequence present in at least 1 - 2% of population
alpha satellite - (answer)highly repetitive sequences at centromere, interfere with chromosome
compaction, causes constriction at centromere
metacentric - (answer)chromosomal arms equal in length
sub-metacentric - (answer)one chromosome arm longer than other
acrocentric - (answer)one chromosome arm extremely small
telocentric - (answer)only 1 chromosome arm
robertsonian translocation - (answer)movement of most of one entire chromosome to centromere of
another
94 - 96°C - (answer)denaturation temp.
50 - 70°C - (answer)annealing temp.
68 - 75°C - (answer)elongation temp.
20 - 30bp - (answer)length of pcr primers
primer dimers - (answer)result from binding of primers onto each other through short (2-3 base)
homologies at 3' ends & copying of each primer sequence
0.1 - 0.5mM - (answer)dNTP concentration for PCR
, ASCP MB Review EXAM (2026) UPDATE Verified Questions And
Answers _ With 100- Correct Answers graded A+ Guaranteed
Pass!!!!
1 - 4mM - (answer)MgCl2 concentration for PCR
positive control - (answer)ensures enzyme is active, buffer is optimal, primers are priming right
sequences, thermocycler is cycling properly
negative control (contamination control, reagent blank) - (answer)ensures reaction mix isn't
contaminated with template DNA or amplicons
negative template control - (answer)lacks target sequence, ensures primers not annealing to nontarget
sequence
amplification control - (answer)2nd set of primers and unrelated target, demonstrates that reaction is
working even if test sample not amplified
nested pcr - (answer)2 sets of primers used to amplify single target in 2 separate pcr runs
semi-nested pcr - (answer)one of 2nd round primers is same as 1st round
taqman probes - (answer)probe with dye at 5' end and quencher at 3' end; exonuclease activity of taq
degrades probe, separating dye and quencher allowing fluorescence
molecular beacons - (answer)measures product at annealing step; stem and loop probe with dye at 5'
end and quencher at 3' end; probe binds to template during annealing and fluoresces
scorpion-type primers - (answer)target-specific primers tailed at 5' end with quencher, stem loop and
fluorophore; after polymerization, secondary structure of primer overcome by hyb of primer to target,
allowing fluorescence
