MIC 206 Inoculation Tests Review With
Complete Solutions
Catalase Input/Output (Catalase Enzyme) - ANSWER Input: Hydrogen Peroxide
Output: Water and Oxygen
Positive result for catalase test shows: - ANSWER Bubbles
Would anaerobes produce catalase? - ANSWER No
Principle of Catalase test - ANSWER Enzyme catalase breaks down hydrogen
peroxide rendering it harmless.
Principle of SIM Citrate test (citrate permease) - ANSWER Determine the ability
of an organism to use sodium citrate (citrate permease) as the sole carbon
source via bromothymol blue pH indicator.
Positive/Negative result for SIM Citrate test shows: - ANSWER Turns blue (>7.6
pH)if positive, if negative- remains green(6.9 pH)
Principle of Malonate test (Malonate) - ANSWER Tests whether an organism can
use malonate as sole carbon source via bromothymol blue pH indicator.
Positive negative result for Malonate Test - ANSWER Turns blue (>7.6 pH)if
positive, if negative- remains green(<6.9 pH)
Principle of Oxidase test (Cytochrome c oxidase) - ANSWER Tests whether
cytochrome c is present within the electron transport chain of the organism.
Positive/Negative result for Oxidase Test - ANSWER Turns purple/blue if positive
and stays clear if negative
Principle of MRVP Broth Methyl Red Test - ANSWER Identifies whether organism
can produce stable acid and products
MRVP Methyl Red Input/Output - ANSWER Input: Glucose
Output: Organic and Mixed Acids (lactate. acetate, succinate, formate)
MRVP Methyl Red Positive/Negative - ANSWER Positive: pink/red
Negative: green/brown
, Vogues-Proskauer (VP) 2,3-Butanediol Fermentation Input/Output - ANSWER
Input: D-Glucose
Output: 2,3-Butanediol
Positive/Negative result Vogues-Proskauer (VP) 2,3-Butanediol Fermentation -
ANSWER Barritt's reagents is used to see the color change. Turns red if positive
and stays copperish color if negative.
If you are Vogues Proskauer Positive then you will be what for Methyl Red? -
ANSWER Negative
Principle of Nitrate Reduction test: - ANSWER Principle = to determine ability of
organism to reduce nitrate to nitrite or free nitrogen gas under anaerobic
conditions
Nitrate Reduction reaction - ANSWER Nitrate is reduced to nitrite and then
reduced to nitric oxide.. Also (NH4) Ammonium is formed.
Reagent used for Nitrate Reduction Test - ANSWER Sulfanilic Acid/dimethyl
alphanapthalamine/Zinc Dust
Positive/Negative results for Nitrate Reduction Test: - ANSWER Positive: Red
color
Negative: Clear or opaque
Phenol Red Test Results - ANSWER red color= neutral
yellow color= acidic
Pink color= alkaline
Fermentation reaction Input/Output - ANSWER Input: Sugar
Output: CO2/Alcohol
A/G - ANSWER Fermentation and gas production
A/- - ANSWER Fermentation, no gas production
-/- - ANSWER No Fermentation and no gas production
K - ANSWER negative carbohydrate fermentation bc basic nature of NH3+
Principle of Phenylalanine Deaminase Test - ANSWER Determines if organisms
can synthesize the enzyme phenylalanine deaminase
Input/Output Phenylalanine Deaminase Test - ANSWER Input: Phenylalanine
Output: Phenylpyruvic acid
Complete Solutions
Catalase Input/Output (Catalase Enzyme) - ANSWER Input: Hydrogen Peroxide
Output: Water and Oxygen
Positive result for catalase test shows: - ANSWER Bubbles
Would anaerobes produce catalase? - ANSWER No
Principle of Catalase test - ANSWER Enzyme catalase breaks down hydrogen
peroxide rendering it harmless.
Principle of SIM Citrate test (citrate permease) - ANSWER Determine the ability
of an organism to use sodium citrate (citrate permease) as the sole carbon
source via bromothymol blue pH indicator.
Positive/Negative result for SIM Citrate test shows: - ANSWER Turns blue (>7.6
pH)if positive, if negative- remains green(6.9 pH)
Principle of Malonate test (Malonate) - ANSWER Tests whether an organism can
use malonate as sole carbon source via bromothymol blue pH indicator.
Positive negative result for Malonate Test - ANSWER Turns blue (>7.6 pH)if
positive, if negative- remains green(<6.9 pH)
Principle of Oxidase test (Cytochrome c oxidase) - ANSWER Tests whether
cytochrome c is present within the electron transport chain of the organism.
Positive/Negative result for Oxidase Test - ANSWER Turns purple/blue if positive
and stays clear if negative
Principle of MRVP Broth Methyl Red Test - ANSWER Identifies whether organism
can produce stable acid and products
MRVP Methyl Red Input/Output - ANSWER Input: Glucose
Output: Organic and Mixed Acids (lactate. acetate, succinate, formate)
MRVP Methyl Red Positive/Negative - ANSWER Positive: pink/red
Negative: green/brown
, Vogues-Proskauer (VP) 2,3-Butanediol Fermentation Input/Output - ANSWER
Input: D-Glucose
Output: 2,3-Butanediol
Positive/Negative result Vogues-Proskauer (VP) 2,3-Butanediol Fermentation -
ANSWER Barritt's reagents is used to see the color change. Turns red if positive
and stays copperish color if negative.
If you are Vogues Proskauer Positive then you will be what for Methyl Red? -
ANSWER Negative
Principle of Nitrate Reduction test: - ANSWER Principle = to determine ability of
organism to reduce nitrate to nitrite or free nitrogen gas under anaerobic
conditions
Nitrate Reduction reaction - ANSWER Nitrate is reduced to nitrite and then
reduced to nitric oxide.. Also (NH4) Ammonium is formed.
Reagent used for Nitrate Reduction Test - ANSWER Sulfanilic Acid/dimethyl
alphanapthalamine/Zinc Dust
Positive/Negative results for Nitrate Reduction Test: - ANSWER Positive: Red
color
Negative: Clear or opaque
Phenol Red Test Results - ANSWER red color= neutral
yellow color= acidic
Pink color= alkaline
Fermentation reaction Input/Output - ANSWER Input: Sugar
Output: CO2/Alcohol
A/G - ANSWER Fermentation and gas production
A/- - ANSWER Fermentation, no gas production
-/- - ANSWER No Fermentation and no gas production
K - ANSWER negative carbohydrate fermentation bc basic nature of NH3+
Principle of Phenylalanine Deaminase Test - ANSWER Determines if organisms
can synthesize the enzyme phenylalanine deaminase
Input/Output Phenylalanine Deaminase Test - ANSWER Input: Phenylalanine
Output: Phenylpyruvic acid
