| 100% Correct Answers | 2026 Updated
📋 TABLE OF CONTENTS
➢ Regulations & IACUC (Questions 1-25)
➢ Ethics & The 3Rs (Questions 26-40)
➢ Animal Husbandry & Environment (Questions 41-55)
➢ Facility Design & Management (Questions 56-70)
➢ Occupational Health & Safety (Questions 71-85)
➢ Species-Specific Care (Questions 86-95)
➢ Technical Procedures (Questions 96-105)
➢ Euthanasia & Humane Endpoints (Questions 106-115)
➢ Answer Key
,SECTION 1: REGULATIONS & IACUC
Questions 1-25, 122-135
Q1. What is research?
• A. A protocol approved by the IACUC
• B. An investigation into facility practices
• C. A systematic investigation to establish facts
• D. A program to help researchers learn new skills
Answer: C
Q2. What is the first step in scientific research?
• A. Create a research design
• B. Define the question to be investigated
• C. Collect data
• D. Report the conclusions
Answer: B
Q3. Which body reviews animal use protocols to ensure compliance with
federal, state, local laws, and institutional policies?
• A. National Institutes of Health (NIH)
• B. United States Department of Agriculture (USDA)
• C. National Science Foundation (NSF)
• D. Institutional Animal Care and Use Committee (IACUC)
Answer: D
Q4. What authority does the IACUC have?
, • A. Approve animal care and use protocols
• B. Deny an investigator permission to begin a research project
• C. Stop a previously approved protocol
• D. All of the above
Answer: D
Q5. IACUCs are mandated by which of the following?
• A. Federal mandate
• B. City ordinance
• C. State law
• D. Institutional policy
Answer: A
Q6. Which mandate requires five IACUC members?
• A. Guide for the Care and Use of Laboratory Animals
• B. Animal Welfare Regulations
• C. Good Laboratory Practice Regulations
• D. Public Health Service (PHS) Policy
Answer: D
Q7. What must occur before a research study using animals begins?
• A. Protocol submitted to IACUC
• B. Protocol received by IACUC
• C. Protocol approved by IACUC
, • D. Protocol examined by IACUC
Answer: C
Q8. How many IACUC members are required by the Animal Welfare Regulations
(USDA)?
• A. 2
• B. 3
• C. 4
• D. 5
Answer: B
Q9. How often does the Guide specify that the IACUC review the entire animal
care program including an inspection?
• A. Every 6 months
• B. At least once a year
• C. Once every 2 years
• D. No less than every 3 years
Answer: A
Q10. The Animal Welfare Regulations require that protocols for USDA-covered
species may only be approved for how long?
• A. 6 months
• B. 1 year
• C. 2 years
• D. 3 years