BCHM 4360 EXAM 2 ACTUAL FINAL
EXAM 2026 WITH QUESTIONS AND
VERIFIED CORRECT ANSWERS |
ALREADY GRADED A+ |
GUARANTEED PASS | BCHM 4360
EXAM II LATEST EXAM [NEWEST]
What is the next step in transcription initiation after TFIID has associated with
DNA? - ANSWER- TFIIB is recruited. This recognizes the BRE promoter element
and binds asymmetrically, helping to determine the transcription direction.
What is the next step in transcription initiation after TFIIB has bound? -
ANSWER- TFIIA binds and stabilizes the TBP-DNA interactions.
What is the next step in transcription initiation after TFIIA binds? - ANSWER-
TFIIE and TFIIH bind, which catalyze ATP-powered unwinding of DNA
What are the two main components of the pre-inititation complex? - ANSWER-
RNA Polymerase II and general transcription factors
True or False: The pre-initiation complex is good enough to initiate transcription in
vitro and in vivo. - ANSWER- False, in vivo requires additional protein complexes
,Another large complex, , is needed to activate many Polymerase II
transcribed genes. - ANSWER- mediator
What are the main similarities between Pol I, II, and III? - ANSWER- Core
subunits are similar, all three use TBP to initiate transcription
What are the main differences between Pol I, II, and III? - ANSWER- TBP is part
of different complexes in each enzyme, each use different proteins to initiate
transcription, recognize different promoters, and have different general
transcription factors
Once RNA Polymerase is in position, the complex of polymerase and promoter is
called a complex. - ANSWER- closed
Approximatel how many bp of duplex DNA (transcription bubble) does RNA
Polymerase open up? - ANSWER- 14bp
True or False: ATP is required to open up the transcription bubble in all three RNA
Polymerases - ANSWER- False, only Pol II requires ATP
The RNA Polymerase bound to an open region of DNA is called the
complex. - ANSWER- open
RNA polymerase frequently fails to make a full length RNA on the first attempt,
which leads to the release of short RNAs of 2-9 nucleotides, and is called
initiation. - ANSWER- abortive
is the key to release the tether attached to RNA Polymerase so
that RNA Polymerase can get going like it wants to. - ANSWER- phosphorylation
The non-template strand of DNA is held apart by which three regions of RNA
Polymerase? - ANSWER- lid, zipper, and rudder regions
, Successive nucleotides are added at the end of the growing RNA molecule by
attack, forming a bond and releasing
. - ANSWER- 3', nucleophilic, phosphodiester, pyrophosphate
The end of the growing RNA leaves the polymerase via an .-
ANSWER- 5', exit channel
Just like DNA Polymerase, which ions are present at the active site and catalyze
the addition of nucleotides? - ANSWER- magnesium
How do magnesium ions work with RNA Polymerase? - ANSWER- activate the 3'
OH and stabilize a negative charge on the leaving oxygen
Bacterial and eukaryotic are involved in abortive initiation.
Both proteins have a that extends into the RNA Polymerase active site region
- ANSWER- sigma factor, TFIIB, loop
What are the three steps of transcription? - ANSWER- initiation, elongation,
termination
How does the loop in abortive initiation function? - ANSWER- It is in position to
block the elongating transcript, so the loop must be moved in order for
transcription to continue. Displacement of the loop is thought to help the
polymerase break away from the promoter - promoter clearance
During promoter clearance, RNA Polymerase undergoes a change
that associates it very with DNA, and its grip on initiation factors.
- ANSWER- conformational, stably, loosens
What is characteristic of the switch from initiation to elongation? - ANSWER-
Dissociation of the general transcription initiation factors
EXAM 2026 WITH QUESTIONS AND
VERIFIED CORRECT ANSWERS |
ALREADY GRADED A+ |
GUARANTEED PASS | BCHM 4360
EXAM II LATEST EXAM [NEWEST]
What is the next step in transcription initiation after TFIID has associated with
DNA? - ANSWER- TFIIB is recruited. This recognizes the BRE promoter element
and binds asymmetrically, helping to determine the transcription direction.
What is the next step in transcription initiation after TFIIB has bound? -
ANSWER- TFIIA binds and stabilizes the TBP-DNA interactions.
What is the next step in transcription initiation after TFIIA binds? - ANSWER-
TFIIE and TFIIH bind, which catalyze ATP-powered unwinding of DNA
What are the two main components of the pre-inititation complex? - ANSWER-
RNA Polymerase II and general transcription factors
True or False: The pre-initiation complex is good enough to initiate transcription in
vitro and in vivo. - ANSWER- False, in vivo requires additional protein complexes
,Another large complex, , is needed to activate many Polymerase II
transcribed genes. - ANSWER- mediator
What are the main similarities between Pol I, II, and III? - ANSWER- Core
subunits are similar, all three use TBP to initiate transcription
What are the main differences between Pol I, II, and III? - ANSWER- TBP is part
of different complexes in each enzyme, each use different proteins to initiate
transcription, recognize different promoters, and have different general
transcription factors
Once RNA Polymerase is in position, the complex of polymerase and promoter is
called a complex. - ANSWER- closed
Approximatel how many bp of duplex DNA (transcription bubble) does RNA
Polymerase open up? - ANSWER- 14bp
True or False: ATP is required to open up the transcription bubble in all three RNA
Polymerases - ANSWER- False, only Pol II requires ATP
The RNA Polymerase bound to an open region of DNA is called the
complex. - ANSWER- open
RNA polymerase frequently fails to make a full length RNA on the first attempt,
which leads to the release of short RNAs of 2-9 nucleotides, and is called
initiation. - ANSWER- abortive
is the key to release the tether attached to RNA Polymerase so
that RNA Polymerase can get going like it wants to. - ANSWER- phosphorylation
The non-template strand of DNA is held apart by which three regions of RNA
Polymerase? - ANSWER- lid, zipper, and rudder regions
, Successive nucleotides are added at the end of the growing RNA molecule by
attack, forming a bond and releasing
. - ANSWER- 3', nucleophilic, phosphodiester, pyrophosphate
The end of the growing RNA leaves the polymerase via an .-
ANSWER- 5', exit channel
Just like DNA Polymerase, which ions are present at the active site and catalyze
the addition of nucleotides? - ANSWER- magnesium
How do magnesium ions work with RNA Polymerase? - ANSWER- activate the 3'
OH and stabilize a negative charge on the leaving oxygen
Bacterial and eukaryotic are involved in abortive initiation.
Both proteins have a that extends into the RNA Polymerase active site region
- ANSWER- sigma factor, TFIIB, loop
What are the three steps of transcription? - ANSWER- initiation, elongation,
termination
How does the loop in abortive initiation function? - ANSWER- It is in position to
block the elongating transcript, so the loop must be moved in order for
transcription to continue. Displacement of the loop is thought to help the
polymerase break away from the promoter - promoter clearance
During promoter clearance, RNA Polymerase undergoes a change
that associates it very with DNA, and its grip on initiation factors.
- ANSWER- conformational, stably, loosens
What is characteristic of the switch from initiation to elongation? - ANSWER-
Dissociation of the general transcription initiation factors
