GMS 6121 Exam 2 Module 2.1
Mononegavirales Latest Update
Common species in Mononegavirales - ANSWER Rabies from Rhabdoviridae
Mumps & Measles, Human respiratory syncytial virus from Paramyxoviridae
What are the fundamental characteristics of Mononegavirales? - ANSWER (-) strand
nonsegmented RNA
enveloped
replication strategy:
has packaged RdRp
RdRp makes viral mRNA inside nucleocapsid after entry
RdRp transcribes 3' end first
sequentially transcribes 5-10 genes
terminates and releases each mRNA before next one
Genome structure of paramyxovirus - ANSWER 15-18kb, rhabdoviruses 11-12kb
3'-->5'
,Leader = signals initiate RNA synthesis
5-10 genes code for specific mRNA, highly conserved
junction between each gene = high conserved
Trailer = UTR, 5' end, noncoding, 50-150nt. Contains promoter for genome replication.
Reason for breaks: RdRp transcribes ORF, gets to genomic break, and terminates.
What do paramyxoviruses look like? - ANSWER Roughly spherical, not as symmetric as
viruses we've viewed before
loose envelope, lipid bilayer
150-300nm virion
Identify components of paramyxovirus cross-layer - ANSWER HN: Hemaglutinnin-
Neuraminidase = receptor binding protein
F: Fusion = fusion protein
N: Nucleocapsid
L:Polymerase
P:Phosphoprotein
SH: Small hydrophobic = don't know fxn well, maybe assemble into pentamers to create
ion channels in membranes = uncoating
, M:Matrix = bridge between envelope glycoproteins and internal nucleocapsid
What are the two main particles of paramyxovirus? - ANSWER HN
F
common theme across viruses, fusion protein only has activity after it has been cleaved,
so F1 and F2 held by disulfide bond = exposes fusion peptide
Describe structure of Paramyxovirus helical nucleocapsid - ANSWER Each N subunit =
6nt in genome
Wound as a 20-nm diameter
left-handed helix
300 copies of P
50 copies of L packaged
Rahbdovirus particles - ANSWER bullet shape
supercoil helical
genome = thread structure complexed with N and Matrix protein
What is the replication cycle of paramyxovirus? - ANSWER Attaches to fusion protein
enters cytoplasm
Mononegavirales Latest Update
Common species in Mononegavirales - ANSWER Rabies from Rhabdoviridae
Mumps & Measles, Human respiratory syncytial virus from Paramyxoviridae
What are the fundamental characteristics of Mononegavirales? - ANSWER (-) strand
nonsegmented RNA
enveloped
replication strategy:
has packaged RdRp
RdRp makes viral mRNA inside nucleocapsid after entry
RdRp transcribes 3' end first
sequentially transcribes 5-10 genes
terminates and releases each mRNA before next one
Genome structure of paramyxovirus - ANSWER 15-18kb, rhabdoviruses 11-12kb
3'-->5'
,Leader = signals initiate RNA synthesis
5-10 genes code for specific mRNA, highly conserved
junction between each gene = high conserved
Trailer = UTR, 5' end, noncoding, 50-150nt. Contains promoter for genome replication.
Reason for breaks: RdRp transcribes ORF, gets to genomic break, and terminates.
What do paramyxoviruses look like? - ANSWER Roughly spherical, not as symmetric as
viruses we've viewed before
loose envelope, lipid bilayer
150-300nm virion
Identify components of paramyxovirus cross-layer - ANSWER HN: Hemaglutinnin-
Neuraminidase = receptor binding protein
F: Fusion = fusion protein
N: Nucleocapsid
L:Polymerase
P:Phosphoprotein
SH: Small hydrophobic = don't know fxn well, maybe assemble into pentamers to create
ion channels in membranes = uncoating
, M:Matrix = bridge between envelope glycoproteins and internal nucleocapsid
What are the two main particles of paramyxovirus? - ANSWER HN
F
common theme across viruses, fusion protein only has activity after it has been cleaved,
so F1 and F2 held by disulfide bond = exposes fusion peptide
Describe structure of Paramyxovirus helical nucleocapsid - ANSWER Each N subunit =
6nt in genome
Wound as a 20-nm diameter
left-handed helix
300 copies of P
50 copies of L packaged
Rahbdovirus particles - ANSWER bullet shape
supercoil helical
genome = thread structure complexed with N and Matrix protein
What is the replication cycle of paramyxovirus? - ANSWER Attaches to fusion protein
enters cytoplasm
