Exam 2 – Microbiology and Protein Structure – 2026/2027 – Review with Well-Answered Questions

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Exam 2 – Microbiology and Protein Structure – 2026/2027 – Review with Well-Answered Questions

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This document contains a 2026/2027 updated Exam 2 review with well-answered questions focused on microbiology techniques and protein biochemistry. It covers replica plating procedures, the Bradford assay, amino acid structure, standard curves, and the four levels of protein organization. The material is structured in a multiple-choice and explanatory format to support concept mastery and exam preparation.

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3/2/26, 11:10
AM

EXAM 2 REVIEW with well answered
questions 2026/2027

1. What is one of the steps to ensure consistency when using the replica

plating technique?

Count the number of colonies before stamping

Make sure apparatus is oriented the same for each plate (north

plate) Perform the control stamp before any test stamping

Use different types of media for each plate

2. What method does the Bradford Assay utilize to measure protein concentration?

Chromatography

Fluorometry

Spectrophotometry

Electrophoresis

3. What does the term 'R group' refer to in the context of amino

acids? Peptide bond structure

Backbone of the amino acid

Variable portion of amino

acid Functional group of a

protein

4. In the context of the standard curve used in microbiology, what does the 'x'

axis typically represent?

https://quizlet.com/test-questions/exam-2-review-exam-71773c80-518e-4414-9932- 1/
2ec3f06a4c64 5

, 3/2/26, 11:10
AM Absorbance

Temperature
Time

5. What determines the charge of proteins?
The amino acids in the polypeptide
chains. The temperature of the
environment.
The pH of the solution.
The presence of metal ions.

https://quizlet.com/test-questions/exam-2-review-exam-71773c80-518e-4414-9932- 2/
2ec3f06a4c64 5

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Uploaded on: March 2, 2026
Number of pages: 5
Written in: 2025/2026
Type: Exam (elaborations)
Contains: Questions & answers

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