Next Generation Sequencing Test Questions and Answers
There are many advantages of using the next errors introduced in replication
generation sequencing and technique as compared
with the Sanger sequencing, for example? -
ANSWER -We can sequence many DNA Now that we have performed the PCR amplification
molecules in parallel and accumulated enough DNA, what is the next
step? - ANSWER -Generating clusters
What are the possible applications of NGS? -
ANSWER -All of these options What is currently happening? - ANSWER -The
DNA molecules are now binding with the adopters On
the flow cell
What is the first step you need to do to prepare the
bone sample for DNA sequencing? -
ANSWER -Extract the DNA How could the DNA molecules bind to the adopter on
the flow cell? - ANSWER -Because the
sequences are complementary
When sequencing ancient DNA, what is the next step
in the sample preparation after we have extracted the
DNA from the bone? - ANSWER -Perform end- What is the name of the process that is currently
repair shown in the animation? - ANSWER -Bridge
PCR
Why do we skip the fragmentation part when we are
sequencing ancient dinner like the sample? - How many DNA molecules are produced in each
ANSWER -Because the DNA is already cluster? - ANSWER -4000
partially degraded
After bridge PCR amplification, half of the DNA
What is the purpose of the end repair procedure? - strands are washed away. Why? - ANSWER -
ANSWER -To create blunt ended strands To sequence clusters of clonal DNA
What is the next step after and repair? - How many clusters do you think we have in one
ANSWER -Adding adenine to the 3' end of the closer? - ANSWER -200 million
Strand
Which enzyme is used in the sequencing process? -
Why do we need an adenine overhang? - ANSWER -T4 DNA polymerase
ANSWER -To ensure that the adaptors bind
specifically
In the next generation sequencing, each of the bases
is tagged with a... So that we can specifically identify
What is the purpose of the adapter? - them. - ANSWER -Fluorophore
ANSWER -For the PCR primers to bind to it
How many pictures are taken for every single base
We only run PCR amplification for 10 - 12 cycles addition - ANSWER -Four
which is much lower than usual 30 cycles. What is
the reason for this? - ANSWER -Minimizing
1/2
There are many advantages of using the next errors introduced in replication
generation sequencing and technique as compared
with the Sanger sequencing, for example? -
ANSWER -We can sequence many DNA Now that we have performed the PCR amplification
molecules in parallel and accumulated enough DNA, what is the next
step? - ANSWER -Generating clusters
What are the possible applications of NGS? -
ANSWER -All of these options What is currently happening? - ANSWER -The
DNA molecules are now binding with the adopters On
the flow cell
What is the first step you need to do to prepare the
bone sample for DNA sequencing? -
ANSWER -Extract the DNA How could the DNA molecules bind to the adopter on
the flow cell? - ANSWER -Because the
sequences are complementary
When sequencing ancient DNA, what is the next step
in the sample preparation after we have extracted the
DNA from the bone? - ANSWER -Perform end- What is the name of the process that is currently
repair shown in the animation? - ANSWER -Bridge
PCR
Why do we skip the fragmentation part when we are
sequencing ancient dinner like the sample? - How many DNA molecules are produced in each
ANSWER -Because the DNA is already cluster? - ANSWER -4000
partially degraded
After bridge PCR amplification, half of the DNA
What is the purpose of the end repair procedure? - strands are washed away. Why? - ANSWER -
ANSWER -To create blunt ended strands To sequence clusters of clonal DNA
What is the next step after and repair? - How many clusters do you think we have in one
ANSWER -Adding adenine to the 3' end of the closer? - ANSWER -200 million
Strand
Which enzyme is used in the sequencing process? -
Why do we need an adenine overhang? - ANSWER -T4 DNA polymerase
ANSWER -To ensure that the adaptors bind
specifically
In the next generation sequencing, each of the bases
is tagged with a... So that we can specifically identify
What is the purpose of the adapter? - them. - ANSWER -Fluorophore
ANSWER -For the PCR primers to bind to it
How many pictures are taken for every single base
We only run PCR amplification for 10 - 12 cycles addition - ANSWER -Four
which is much lower than usual 30 cycles. What is
the reason for this? - ANSWER -Minimizing
1/2
